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| 不同nPCL/HA电纺纤维薄膜对人骨髓间充质干细胞黏附率的影响 | |
| 引用本文： | 李家锋,万美蓉,管海虹,万延俊,贺文鹏,张扬,陈丽娟. 不同nPCL/HA电纺纤维薄膜对人骨髓间充质干细胞黏附率的影响[J]. 中国美容医学, 2009, 18(9): 1302-1305 |
| 作者姓名： | 李家锋 万美蓉 管海虹 万延俊 贺文鹏 张扬 陈丽娟 |
| 作者单位： | 1. 徐州医学院附属徐州市立医院口腔颌面外科,江苏,徐州,221002 2. 徐州医学院附属医院细胞学实验室 |
| 摘 要： | 目的：观察nPCL/HA电纺纤维取向薄膜材料对人骨髓间充质干细胞（hBMSCs）的黏附生长的影响,评价其作为组织工程支架材料的应用前景。方法：将体外诱导培养的人骨髓间充质干细胞（hBMSCs）,经传代培养第5代的人骨髓间充质干细胞,以2×105的密度与不同nPCL/HA电纺纤维取向薄膜支架在培养板内共培养,同时以nPCL电纺纤维非取向薄膜材料作为对照,初步观察不同nPCL/HA支架材料与hBMSCs的复合培养情况,计算各材料的细胞黏附率。结果：hBMSCs与4种电纺薄膜支架材料均有良好的细胞相容性,细胞能在不同材料表面黏附生长。但是nPCL/80℃HA电纺纤维取向薄膜材料黏附率（45.6±3.2）%,明显高于nPCL/180℃HA电纺纤维取向薄膜材料黏附率（36.5±3.0）%。结论：80℃条件下合成的nPCL/HA电纺取向薄膜材料细胞黏附率较高,较适合作为支架材料应用于hBMSCs为种子细胞的组织工程构建。 |
| 关 键 词： | 骨髓间充质干细胞 聚己内酯 羟基磷灰石 细胞黏附 |
Effect of various electrospun nPCL/HA scaffold materials on the adhesion rate of hBMSCs |
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| LI Jia-feng,WAN Mei-rong,GUAN Hai-hong,WAN Yan-jun,HE Wen-peng,ZHANG Yang,CHEN Li-juan. Effect of various electrospun nPCL/HA scaffold materials on the adhesion rate of hBMSCs[J]. Chinese Journal of Aesthetic Medicine, 2009, 18(9): 1302-1305 | |
| Authors: | LI Jia-feng WAN Mei-rong GUAN Hai-hong WAN Yan-jun HE Wen-peng ZHANG Yang CHEN Li-juan |
| Affiliation: | 1.Department of Oral Maxillofacial Surgery;Affiliated Xuzhou City Hospital of Xuzhou Medical College;2.Affiliated Hospital of Xuzhou Medical College;Xuzhou 221002;Jiangsu;China |
| Abstract: | Objective To investigate the effect of different nPCL/HA scaffold materials on the adhesive growth of hBMSCs.Methods Electrostatic spinning or electrospinning is an interesting method for producing nonwoven fibers with diameters of submicrometers down to nanometers.Nanofibrous membranes were used in many biomedical applications including drug delivery,wound healing and scaffolding for tissue engineering.Novel bone-scaffolding materials were successfully fabricated by electrospinning from polycaprolactone(PC... |
| Keywords: | bone marrow stromal cells polycaprolactone hydroxypatite cell adhesion |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |