Bilateral vagotomy attenuates the severity of secretagogue-induced acute pancreatitis in the rat |
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| Institution: | 1. Department of Medical Physiology, Health Care Faculty, Jagiellonian University Collegium Medicum, Krakow, Poland;2. Department of Cell Morphology Medical Faculty, Jagiellonian University Collegium Medicum, Krakow, Poland;1. Department of Digestive Diseases and Internal Medicine, Sant’Orsola-Malpighi Hospital, University of Bologna, Bologna, Italy;2. Department of Surgery, GB Rossi Hospital, University of Verona, Verona, Italy;3. Department of Pancreatic Surgery, S. Raffaele Hospital, Milan, Italy;4. Department of General Surgery, S. Orsola-Malpighi Hospital, University of Bologna, Bologna, Italy;1. Department of Clinical Pathomorphology, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Torun, Bydgoszcz, Poland;2. Department of Otolaryngology and Clinical Oncology Chair and Clinic of Otolaryngology and Department of Pathophysiology of Hearing and Balance System Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Torun, Bydgoszcz, Poland;3. Department of Oncologic Pathology, Poznan University of Medical Sciences and Greater Poland Oncology Center, Poznan, Poland;1. 2nd Department of Internal Medicine, University of Medicine and Pharmacy “Iuliu Hatieganu”, Cluj-Napoca, Romania;2. Medical Informatics and Biostatistics Department, University of Medicine and Pharmacy “Iuliu Hatieganu”, Cluj-Napoca, Romania;3. Biochemistry Department, University of Medicine and Pharmacy “Iuliu Hatieganu”, Cluj-Napoca, Romania;1. I Department of Cardiology, Invasive Electrocardiology and Hypertension, Jagiellonian University Medical College, University, Krakow, Poland;2. Department of Invasive Cardiology, Electrotherapy and Angiology, Nowy Sacz Hospital, Nowy Sacz, Poland |
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| Abstract: | PurposeWe assessed the effect of bilateral vagotomy (BV) on the course of acute caerulein-induced pancreatitis (AP) in the rat.Material/methodsThe study was performed on Wistar rats surgically prepared by subdiaphragmatic BV. Control group underwent sham operation. Four days later, AP was induced by subcutaneous injection of caerulein (25 μg/kg/5 h) to the conscious animals with or without BV. After administration of caerulein the blood samples were taken for determination of serum lipase activity and interleukin-10 (IL-10) concentration. Pancreatic tissue samples were subjected to histological examinations and to the measurement of lipid peroxidation products (MDA + 4-HNE) concentration and the activity of an antioxidant enzyme – glutathione peroxidase (GPx). After application of caerulein pancreatic blood flow was measured by laser Doppler flowmetry.ResultsAP was manifested by oedema and neutrophil infiltration of the pancreatic tissue and accompanied by significant increases of serum lipase activity, serum concentration of IL-10 and pancreatic concentration of MDA + 4HNE (ca. 50×, 2× and 4× respectively p ≥ 0.05). Pancreatic activity of GPx and pancreatic blood flow were decreased (both by 60%). In vagotomised rats with AP serum lipase activity and pancreatic concentration of MDA + 4-HNE were lower whereas Il-10 concentration and pancreatic activity of GPx, as well as pancreatic blood flow were significantly higher as compared to AP rats with intact vagal nerves. In AP rats with vagotomy all histological signs of pancreatitis were significantly reduced.ConclusionsBilateral vagotomy resulted in the significant attenuation of caerulein-induced pancreatitis in the rat. |
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| Keywords: | Bilateral vagotomy Caerulein-induced pancreatitis Pancreatic blood flow Oxidative stress Lipase Interleukin 10 |
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