荷叶中化学成分的分离与鉴定

目的研究荷叶中的化学成分。方法采用正相硅胶、反相ODS、Sephadex LH-20等柱色谱及PHPLC法进行分离纯化,并通过理化性质、光谱分析方法及与文献对比,鉴定化合物的化学结构。结果从荷叶体积分数为70%的乙醇提取物中分离鉴定了8个化合物,其中有4个木脂素类:(+)-松脂醇(+)-pinoresinol,1]、(+)-表松脂醇(+)-epipinoresinol,2]、sylvatesmin(3)、(+)-异落叶松树脂醇(+)-isolariciresinol,4];4个降倍半萜类:(3S,5R,6S,7E)-5,6-epoxy-3-hydroxy-7-me-gastigmen-9-one(5)、4,5-dihydroblumenol A(6)、(E)-3-oxo-retro-α-ionol(7)、(3S,5R,6R,7E,9S)-megastigman-7-ene-3,5,6,9-tetraol(8)。结论化合物1-8均为首次从莲属植物中分离得到。

Isolation and identification of chemical constituents from the leaves of Nelumbo nucifera Gaertn.

Objective To study the chemical constituents of the leaves of Nelumbo nucifera Gaertn. Methods The compounds were isolated by silica gel, ODS, Sephadex LH-20 column chromatography and PHPLC, and their structures identified by physico-chemical properties, spectral analysis and literatures. Results Four lignanoids and four C₁₃ nor-isoprenoids were isolated from 70% ethanol extract of lotus leaves and identified as (+)-pinoresinol(1), (+)-epipinoresinol (2), sylvatesmin (3), (+)-isolariciresinol (4), (3S,5R,6S,7E)-5,6-epoxy-3-hydroxy-7-megastigmen-9-one (5), 4,5-dihydroblumenol A (6), (E)-3-oxo-retro-α-ionol (7), (3S,5R,6R,7E,9S)-megastigman-7-ene-3,5,6,9-tetraol (8), respectively. Conclusions Compounds 1-8 are obtained from Nelumbo Adans. for the first time.