中国北方汉族急性白血病和慢性粒细胞白血病患者HLA等位基因及单倍型研究

目的 研究中国北方汉族急性白血病(AL)和慢性粒细胞白血病(CML)患者人群中人类白细胞抗原HLA-A、B、DRB1等位基因和单倍型的分布差异.方法 根据1 270例中国北方汉族AL病患者和803例中国北方汉族CML患者的HLA-A、B、DRB1表型数据,采用最大似然性方法分别计算两个群体的HLA-A、B、DRB1等位基因和单倍型频率,并采用(2检验方法比较其分布差异.结果 两个患者群体中,A位点的A*02、A*03、A*24,B位点的B*37、B*38、B*44、B*45、B*46、B*50、B*51、B*52、B*54、B*60、B*65,DR位点的DRB1*03、DRB1*09、DRB1*10、DRB1*15等位基因的分布有统计学差异.在两个患者群体中有326条A-B单倍型,357条B-DRB1单倍型,1 278条A-B-DRB1单倍型为共有单倍型,其中7.1%(23/326)A-B单倍型,6.2%(22/357)B-DRB1单倍型,4.0%(51/1278)条A-B-DRB1单倍型有统计学差异(x2>3.84,P<0.05).结论 中国北方汉族AL和CML两个患者群体的HLA-A、B、DRB1等位基因和单倍型均具有高度遗传多态性,并有其自身遗传特征.

Polymorphism of HLA-A,HLA-B and DRB1 alleles and haplotypes in Chinese northern Han patients with acute leukemia and chronic myeloid leukemia

Objective To study the polymorphism of HLA-A,B,DRB1 alleles and haplotypes in Chinese northern Han patients with AL and CML.Methods The frequencies of HLA-A,B,DRB1 alleles and haplotypes were estimated by Expectation-Maximization method based on the phenotypes of 1 270 AL patients and 803 CML patients in Chinese northern Han,and then compared by chi-square test.Results The frequent of A*02,A*03,A*24,B*37,B*38,B*44,B*45,B*46,B*50,B*51,B*52,B*54,B*60,B*65,DRB1*03,DRB1*09,DRB1*10 and DRB1*15 alleles were different with statistically significant in both AL and CML patients in Chinese northern Han.326 HLA-A-B haplotypes,357 HLA-B-DRB1 haplotypes and 1 278 HLA-A-B-DRBl haplotypes were the common haplotype in the two groups of patients.7.1%(23/326)of HLA A-B haplotypes,6.2%(22/357)of HLA-B-DRB1 haplotypes and 4.0%(51/1278)of HLA-A-B DRB1 haplotypes were different with statistically significant in the two groups of patients.Conclusion The distribution of HLA-A,B,DRB1 alleles and haplotypes has rich polymorphism and has own genetic characteristics in Chinese northern Han patients with AL and CML.