啤酒酵母RNA对5-氟尿嘧啶处理小鼠免疫功能的影响

目的 探讨啤酒酵母RNA对5-氟尿嘧啶(5-Fu)处理小鼠免疫功能的影响.方法 将小鼠随机分为空白组,5-氟尿嘧啶组,啤酒酵母RNA小剂量组、大剂量组分别以啤酒酵母RNA 100 mg/(kg·d)、300 mg/(kg·d)灌胃给药];除空白组外,其余各组均以5-Fu 25 mg/(kg·d)灌胃.检测腹腔巨噬细胞吞噬率,血清溶血素形成水平、肿瘤坏死因子-α(TNF-α)及白细胞介素-2(IL-2)的含量和淋巴细胞转化率,计算脾指数及胸腺指数.结果 啤酒酵母RNA大、小剂量组均可明显提高5-氟尿嘧啶处理小鼠腹腔巨噬细胞吞噬率、血清TNF-α、IL-2水平,逆转脾指数、胸腺指数的萎缩,与5-氟尿嘧啶组相比差异具有统计学意义(P<0.05或P<0.01);啤酒酵母RNA大、小剂量组对5-氟尿嘧啶所致的血清溶血素形成水平低下和淋巴细胞转化率低下有明显的拮抗作用(P<0.05或P<0.01).结论 啤酒酵母RNA可提高免疫功能低下机体的免疫功能.

Effects of Brewer's Yeast RNA on Immune Function in Mice Treated with 5-Fluorouracil

Objective To investigate the effects of brewer's yeast RNA on immune insufficiency in mice treated with 5-flu-orouracil. Methods The spleen and thymus index.phagocytosis rate of peritoneal macrophages,levels of serum hemolysin,contents of serum TNF-α and IL-2,and lymphocyte transformation rate were measured after mice were treated with 5-fluorouracil 25 mg/(kg ? d)] and/or brewer's yeast RNA 100 mg/(kg ? d) ,300 mg/(kg ? d) ,respectively] for 14 days. Results The phagocytosis rate of peritoneal macrophages, levels of serum TNF-α and IL-2. and spleen and thymus index in brewer' s yeast RNA-treated groups were significantly higher than those in 5-fluorouracil-treated group (P<0. 05,or P<0. 01). As compared with 5-fluorouracil-treated group,the levels of serum hemolysin and lymphocyte transformation rate were notably increased in brewer's yeast RNA-treated groups (P<0. 05,or P<0. 01). Conclusion Brewer's yeast RNA may increase the immune function of the mice with immune insufficiency.