Diagnosis of pulmonary infection with Toxoplasma gondii in immunocompromised HIV-positive patients by real-time PCR |
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| Authors: | E. Petersen B. Edvinsson B. Lundgren T. Benfield B. Evengård |
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| Affiliation: | (1) Department of Clinical Bacteriology F82, Karolinska Institutet at Karolinska University Hospital—Huddinge, 14186 Stockholm, Sweden;(2) Department of Infectious Diseases, Aarhus University Hospital—Skejby, 8200 Aarhus N, Denmark;(3) Department of Laboratory Medicine F82, Karolinska Institute at Karolinska University Hospital—Huddinge, 14186 Stockholm, Sweden;(4) Department of Infectious Diseases and Copenhagen HIV Programme, Hvidovre University Hospital—Hvidovre, 2650 Hvidovre, Denmark;(5) Department of Clinical Microbiology, Hvidovre University Hospital—Hvidovre, 2650 Hvidovre, Denmark |
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| Abstract: | ![]()
The aim of the study presented here was to evaluate the use of PCR for improving the diagnosis of Toxoplasma gondii infection in immunocompromised hosts. Three hundred thirty-two bronchoalveolar lavage (BAL) fluid samples were analyzed by real-time PCR targeting a 529 bp element of T. gondii. In positive samples, the genotype of the parasite was determined by sequence analysis of the GRA6 gene. Positive results were achieved for 2% (7/332) of the samples tested. Genotyping was possible in two samples and revealed GRA6 type II T. gondii. PCR for detecting T. gondii in BAL samples should be performed in all immunosuppressed HIV-positive patients with symptoms of a systemic infection of unknown etiology. Trimethoprim-sulfamethoxazole prophylaxis does not exclude concomitant infection with T. gondii. |
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