End-group effects on the properties of PEG-co-PGA hydrogels |
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| Authors: | Sidi A. Bencherif Abiraman Srinivasan Jeffrey A. Sheehan Lynn M. Walker Chakicherla Gayathri Roberto Gil Jeffrey O. Hollinger Krzysztof Matyjaszewski Newell R. Washburn |
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| Affiliation: | 1. Department of Chemistry, Carnegie Mellon University, Pittsburgh, PA 15213, USA;2. Bone Tissue Engineering Center, Carnegie Mellon University, Pittsburgh, PA 15213, USA;3. Department of Chemical Engineering, Carnegie Mellon University, Pittsburgh, PA 15213, USA;4. Department of Biomedical Engineering, Carnegie Mellon University, Pittsburgh, PA 15213, USA;1. Biomedical Polymers Laboratory, and Jiangsu Key Laboratory of Advanced Functional Polymer Design and Application, College of Chemistry, Chemical Engineering and Materials Science, Soochow University, Suzhou 215123, People’s Republic of China;2. Cardiovascular Surgery Department, The First Affiliated Hospital of Soochow University, Suzhou 215012, People’s Republic of China;3. Division of Cardiac Surgery, University of Ottawa Heart Institute, Ottawa K1Y 4W7, Canada;1. 3B’s Research Group – Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, Avepark – Parque de Ciência e Tecnologia, Zona Industrial da Gandra, 4805-017 Barco GMR, Guimarães, Portugal;2. ICVS/3B’s – PT Government Associate Laboratory, Braga/Guimarães, Portugal;3. CNRS, UMR 5628, LMGP, 3 parvis Louis Néel, F-38016 Grenoble, France;4. Université de Grenoble Alpes, Grenoble Institute of Technology, 3 parvis Louis Néel, F-38016 Grenoble, France;5. Institute Albert Bonniot, INSERM U823, ERL CNRS3148, Grenoble, France;1. Dept. of Bioengineering, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA;2. Dept. Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA;3. Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA;1. Department of Biomedical Engineering, Texas A&M University, College Station, TX 77843, United States;2. Department of Materials Science and Engineering, Texas A&M University, College Station, TX 77843, United States |
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| Abstract: | ![]()
A series of resorbable poly(ethylene glycol)-co-poly(glycolic acid) (PEG-co-PGA, 4KG5) macromonomers have been synthesized with the chemistries from three different photopolymerizable end-groups (acrylates, methacrylates and urethane methacrylates). The aim of the study is to examine the effects of the chemistry of the cross-linker group on the properties of photocross-linked hydrogels. 4KG5 hydrogels were prepared by photopolymerization with high vinyl group conversion as confirmed by 1H nuclear magnetic resonance spectrometry using a 1D diffusion-ordered spectrometry pulse sequence. Our study reveals that the nature of end-groups in a moderately amphiphilic polymer can adjust the distribution and size of the micellar configuration in water, leading to changes in the macroscopic structure of hydrogels. By varying the chemistry of the cross-linker group (diacrylates (DA), dimethacrylates (DM) and urethane dimethacrylates (UDM)), we determined that the hydrophobicity of a single core polymer consisting of poly(glycolic acid) could be fine-tuned, leading to significant variations in the mechanical, swelling and degradation properties of the gels. In addition, the effects of cross-linker chemistry on cytotoxicity and proliferation were examined. Cytotoxicity assays showed that the three types of hydrogels (4KG5 DA, DM and UDM) were biocompatible and the introduction of RGD ligand enhanced cell adhesion. However, differences in gel properties and stability differentially affected the spreading and proliferation of myoblast C2C12 cells. |
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