溴苯腈急性中毒小鼠脑组织能量代谢的改变

目的通过检测溴苯腈急性中毒时小鼠脑组织能量代谢的变化，以探讨溴苯腈急性中毒可能的毒理机制。方法将16只ICR小鼠随机分为正常对照组与溴苯腈灌胃染毒急性中毒组，采用反相高效液相色谱法测定两组小鼠脑组织三磷酸腺苷酸（ATP）、二磷酸腺苷酸（ADP）、一磷酸腺苷酸（AMP）含量，并计算总腺苷酸（TAN）含量及细胞能荷（EC）值；同时采用分光光度法测定脑组织钠钾腺苷三磷酸酶（Na^＋-K^＋-ATPase）和钙镁腺苷三磷酸酶（Ca^2＋-Mg^2＋-ATPase）活性。结果急性中毒组脑组织ATP、TAN含量、EC水平和Na^＋-K^＋-ATPase、Ca^2＋-Mg^2＋-ATPase活性均明显低于正常对照组（均P〈0．05或0．01）。AMP含量明显高于正常对照组（P〈0．01）；两组脑组织ADP含量的差异无统计学意义（P〉0．05）。结论溴苯腈急性中毒可导致小鼠脑组织能量代谢障碍，可能是溴苯腈急性中毒的中枢毒理机制之一。

Changes in brain energy metabolism after acute bromoxynil intoxication in mice

Objective To investigate the changes of brain energy metabolism following acute bromoxynil intoxication in mice. Methods Sixteen ICR mice were randomly divided into control group and acute bromoxynil intoxication （ABI） group （n=8）. The contents of adenosine triphosphate （ATP）, adenosine diphosphate （ADP） and adenosine monophosphate （AMP） in the brain tissue were measured by RP-HPLC, and the values of total adenine nucleotides （TAN） and energy charge （EC） were calculated; the activities of Na^＋-K^＋-ATPase and Ca^2＋-Mg^2＋-ATPase in brain tissue were assayed by spectrophotometric method. Results Jn ABI group, the contents of ATP, the level of EC, the activities of Na^＋-K^＋-ATPase and Ca^2＋-Mg^2＋-ATPase in the brain tissue were significantly lower than those in control group （P〈0.05-0.01）;AMP contents of brain tissue in ABI group were higher than those in control group （P〈0.01）;The difference between the ADP contents of brain tissue between two groups was not statistically significant （P〉0.05）. Conclusion Energy metabolism disorder in brain tissue may be one of the pathogenic mechanisms of acute bromoxynil intoxication.