| The Apoptosis of Bovine Lens Epithelial Cells Induced by Proteasome Inhibitor MG132 |
| |
| 引用本文： | 邢星,胡义珍,曹阳,徐志蓉. The Apoptosis of Bovine Lens Epithelial Cells Induced by Proteasome Inhibitor MG132[J]. 华中科技大学学报(医学英德文版), 2008, 28(4): 469-471. DOI: 10.1007/s11596-008-0421-1 |
| |
| 作者姓名： | 邢星 胡义珍 曹阳 徐志蓉 |
| |
| 作者单位： | Department of Ophthalmology，Union Hospital，Tongji Medical College，Huazhong University of Science and Technology |
| |
| 摘 要： | To investigate the effect of proteasome inhibitor MG132 on the apoptosis of bovine lens epithelial cells （BLECs）, the cells were treated with MG132 at different concentrations for12, 24 and 36 h. The cell viability was analyzed by MTT assay and the effect of MG132 on the apoptosis of BLECs was assessed by flow cytometry （FCM）. The results showed that after treatment for the same period, the inhibitory effect of MG132 on BLECs proliferation was enhanced with the increment of the concentration of MG132 （0, 2, 5, 10, μmol/L） （P〈0.05）. The 50% inhibiting concentration （IC50） was 2.03 μmol/L when the BLECs were treated with MG132 for 36 h. MG132 also induced the apop- tosis of BLECs obviously. FCM showed that the apoptosis index of the cells treated by MG132 at 2 μmol/L for 12 h was （20.24±1.51）%, and that of the control was （0.98±0.20）% respectively （P〈0.01, n=3）. It was concluded that MG132 could lead to apoptosis of BLECs. The decrease of proteasome activity may play an important role in the formation and development of cataract.
|
| 关 键 词： | 晶状体内皮细胞 白内障 细胞凋亡 MG132 |
| 收稿时间： | 2009-01-28 |
The apoptosis of bovine lens epithelial cells induced by proteasome inhibitor MG132 |
| |
| Xing Xing,Yizhen Hu,Yang Cao,Zhirong Xu. The apoptosis of bovine lens epithelial cells induced by proteasome inhibitor MG132[J]. Journal of Huazhong University of Science and Technology. Medical sciences, 2008, 28(4): 469-471. DOI: 10.1007/s11596-008-0421-1 |
| |
| Authors: | Xing Xing Yizhen Hu Yang Cao Zhirong Xu |
| |
| Affiliation: | Department of Ophthalmology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China |
| |
| Abstract: | To investigate the effect of proteasome inhibitor MG132 on the apoptosis of bovine lens epithelial cells (BLECs), the cells were treated with MG132 at different concentrations for12, 24 and 36 h. The cell viability was analyzed by MTT assay and the effect of MG132 on the apoptosis of BLECs was assessed by flow cytometry (FCM). The results showed that after treatment for the same period, the inhibitory effect of MG132 on BLECs proliferation was enhanced with the increment of the concentration of MG132 (0, 2, 5, 10, mumol/L) (P<0.05). The 50% inhibiting concentration (IC(50)) was 2.03 mumol/L when the BLECs were treated with MG132 for 36 h. MG132 also induced the apoptosis of BLECs obviously. FCM showed that the apoptosis index of the cells treated by MG132 at 2 mumol/L for 12 h was (20.24+/-1.51)%, and that of the control was (0.98+/-0.20)% respectively (P<0.01, n=3). It was concluded that MG132 could lead to apoptosis of BLECs. The decrease of proteasome activity may play an important role in the formation and development of cataract. |
| |
| Keywords: | proteasome inhibitor lens epithelial cells cataract apoptosis |
| 本文献已被 CNKI 维普 万方数据 SpringerLink 等数据库收录！ |
|
