异源双链鉴定法检测淋巴瘤IgH、TCR-β基因重排

目的建立更敏感、特异性更强的PCR方法检测淋巴瘤IgH和，TCR-β基因重排。方法采用标准PCR方法以及异源双链鉴定的改良方法检测55例淋巴瘤组织，全部病例均经组织学明确诊断，其中36例为B细胞性淋巴瘤，19例为T细胞性淋巴瘤。对照为5例反应性增生的淋巴结组织。结果用改良PCR方法检测淋巴瘤组IgH和TCR-β基因重排49／55例为阳性，较标准PCR方法电泳条带更清晰、更易于判定；5例反应性增生的淋巴结组织均为阴性。结论异源双链鉴定的改良方法提高了检测淋巴组织肿瘤性增生的敏感性和特异性，值得推广。

Detection of clonal immunoglobulin heavy chain and TCR-β gene rearrangements in paraffin embedded lymphoma tissues by polymerase chain reaction

Objective Molecular detection of a clonal population of B or T cells by analyzing rearrangement of immunoglobulin heavy chain (IgH) and T cell receptor (TCR) is an essential adjunct to the morphology of lymphatic tissue. To detect clonal rearrangements of IgH gene and TCR gene more efficiently, we used a technique of combining polymerase chain reaction (PCR) with heteroduplex annealing and PAGE. Methods Fifty-five cases of lymphoma including 36 cases of B cell lymphoma and 19 cases of T cell lymphoma, were investigated with combining PCR with heteroduplex annealing and PAGE. Five cases of reactively proliferative lymph nodes were set up as a control. Results Forty-nine of 55 cases of lymphoma in this heteroduplex analysis were showing a sharper positive electrophoresis band than that in conventional method and five cases of reactively proliferative lymph nodes were negative. It could be used in paraffin-embedded tissue to detect a clonal population in a proliferative background. Compared with conventional PCR, this method needed only a short additional denaturation and slow renaturation before PAGE. Interpretation was simplified as the clonal PCR product migrated away from the polyclonal background products. Conclusion This heteroduplex analysis method can be used to detect a monoclonal rearrangement in a polyclonal background with more sensitivity and specialty than conventional one.