糖皮质激素对人卵巢癌细胞系3AO增殖分化及对糖皮质激素受体表达的影响

目的：观察不同浓度地塞米松（Dex)对人卵巢癌细胞系（3AO）增殖及分化的影响及其对糖皮质激素受体（GR）的调节作用。方法：以不同浓度Dex处理3AO细胞,采用活细胞计数法观察细胞增殖,用氨基安替比林法测定碱性磷酸酶（AKP）活性;用酶联免疫法测定细胞标志抗原CA125水平的变化;用放射配体结合法测定GR的表达。结果：Dex对3AO细胞的增殖有抑制作用,同时伴有细胞形态的变化及AKP活性增高和CA125的表达下降。Dex对3AO细胞AKP活性的诱导作用可被RU486所阻断。在3AO细胞中存在糖皮质激素受体（GR）,Dex对GR结合活性有下调作用。结论：Dex对3AO细胞有增殖抑制和诱导分化作用,这种作用是通过GR来介导的。

Effects of glucocorticoid on proliferation,differentiation, and glucocorticoid receptor expression in human ovarian carcinoma cell line 3AO

AIM: To study the effects of dexamethasone (Dex), a synthetic glucocorticoid, on proliferation, differentiation, glucocorticoid receptor expression and regulation in human ovarian cancer cell line 3AO. METHODS: 3AO cells proliferation was evaluated by viable cell count, activity of alkaline phosphatase (AKP) and tumor marker CA125 level were determined; the expression and regulation of glucocorticoid receptor (GR) in 3AO cells was studied with radioligand binding assay. RESULTS: Dex inhibited the proliferation of 3AO cells accompanied by morphological changes in concentration- and time- dependent manner. AKP activity was increased and tumor marker CA125 was decreased in 3AO cells after treatment with Dex. The induction of AKP activity by dexamethasone was blocked by RU486, a potent glucocorticoid antagonist. There existed high affinity and low capacity of GR in 3AO cells, and the GR binding activity could be downregulated by Dex. CONCLUSION: Glucocorticoids play an important role in the regulation of 3AO cell proliferation and differentiation. There existed functional GR in 3AO cells and the cellular effects of dexamethasone on 3AO cells were mediated by GR.