| 卵巢癌及癌旁组织端粒酶活性与凋亡相关性研究 |
| |
| 引用本文： | 张汉英,;黎丹戎,;张惠煊,;丁敏,;游华蓉. 卵巢癌及癌旁组织端粒酶活性与凋亡相关性研究[J]. 江苏临床医学杂志, 2009, 0(4): 42-45 |
| |
| 作者姓名： | 张汉英, 黎丹戎, 张惠煊, 丁敏, 游华蓉 |
| |
| 作者单位： | [1]广东省深圳市宝安区龙华医院,深圳宝安518109; [2]广西医科大学附属肿瘤医院,广西南宁530021; [3]广东省深圳市东莞市东坑医院,广东东莞523451; [4]广东省中山市南朗医院,广东中山528400 |
| |
| 摘 要： | 目的研究端粒酶活性和抗凋亡基因Bel-2及促凋亡基因Bax在卵巢癌发生、发展过程中的变化。方法应用SP免疫组织化学方法,对50例卵巢癌及癌旁正常组织进行hTERT、Bcl-2、Bax基因蛋白的检测。结果hTERT和Bcl-2阳性表达率在卵巢癌组织中高于癌旁组织。而Bax阳性表达率在卵巢癌组织低于癌旁组织,2组间均有明显差异。hTERT蛋白表达阳性率在临床Ⅰ、Ⅱ期和临床Ⅲ、Ⅳ期分别为16／23（69．5696）和26／27（96．30％）,差异有显著性（P〈0．05）。高分化组织与低分化组织中阳性率分别为18／23（78．26％）和25／27（92．59％）,差异也有显著性（P〈0．05）。对于Bcl-2蛋白表达阳性率在临床Ⅰ、Ⅱ期和Ⅲ、Ⅳ期中分别为8／23（34．78％）和16／27（59．26％）,高分化组织和低分化组织阳性率分别为9/23（39．13％）和18／27（66．67％）。差异均有显著性（P〈0．05）。卵巢癌组织凋亡指数明显低于卵巢癌旁组织,差异有显著性（P〈0．05）。结论凋亡调控功能失调与肿瘤的形成有关,hTERT、Bel-2、Bax基因蛋白的异常表达与卵巢癌发生有关;hTERT、Bel-2在卵巢癌的发生发展中可能有协同作用,hTERT、Bel-2与Bax在卵巢癌的发生发展中可能有拮抗作用。hTERT、Bel-2蛋白的表达可能是反映卵巢癌生物学行为的重要参数。
|
| 关 键 词： | 卵巢癌 端粒酶 hTERT基因 Bcl-2基因 Bax基因 凋亡 |
A study on correlation between ovarian cancer telomerase activity and apoptosis |
| |
| Affiliation: | ZHANG Han-ying, LI Dan-rong, ZHANG Hui-xuan, DING Min, YOU Hua-rong (1. Longhua Hospital o f Ban＇an District, Shenzhen , Guangdong , 518109; 2. Tumor Hospital Affiliated to Guangxi Medical University, Nanning , Guangxi, 530021 ; 3. Dongkeng Hospital, Dongguan , Guangdong , 523451 ; 4. Nanlang Hospital, Zhongshan city, Guangdong , 528400) |
| |
| Abstract: | Objective To study the change of telomerase activation and anti-apoptosis gene Bcl-2 and apoptosis gene Bax during the process of ovarian cancer. Methods immunohistochemistry was used as the method, hTERT, Bcl-2, and Bax of 50 cases of ovaries and adjacent tissues were examined. Results The positive rate of hTER and Bcl-2 was higher than those of adjacent tisse. While positive rate of Bax in ovarian cancer was lower than that in the adjacent tissues, the difference was significant, the expression rate of hTERT 69.56 % in clinical phase Ⅰ , Ⅱ and 96.30 % in clinical phase of Ⅲ, Ⅳ demonstrated the difference was significant. The rates of hTERT in welldifferentiated and poorly differentiated tissues were 78.26 % and 92.59 %. The difference was significant. The expression rate of Bcl-2 in clinical phase Ⅰ , Ⅱ and clinical phase Ⅲ, Ⅳ was 34.78 % and 59.26% respectively, the positive rate of well-differentiated and poorly differentiated tissues were 39.13 % and 66.67% respectively. The difference was significant. Apoptotic index of ovarian cancer was lower than that of the adjacent tissues. Conclusions Functional disorder of apoptosis was related to the tumor. The abnormal expressions of hTERT, Bcl-2 and Bax were related to the genesis of ovarian cancer. While hTERT and Bcl-2 was conducive to the tumor, hTERT, Bcl-2 and Bax can prevent the genesis of ovarian cancer. The expressions of hTERT and Bcl-2 were important parameter that reflects the biological character of ovarian cancer |
| |
| Keywords: | ovarian cancer telomerase hTERT gene apoptosis |
| 本文献已被 维普 等数据库收录！ |
|
