诱导型一氧化氮合酶在实验性病毒性心肌炎心肌内的表达及其与心肌损害的关系

目的 探讨诱导型一氧化氮合酶（ｉＮＯＳ）在急性病毒性心肌炎心肌内的表达及其与心肌损害的关系。方法 ６～８周龄ＢＡＬＢ／Ｃ雄性小鼠２４只，随机分两组：感染组１６只以柯萨奇病毒Ｂ３ｍ（ＣＶＢ３ｍ）腹腔内注射，另８只为正常对照组。采用免疫组化法检测两组小鼠心肌组织内ｉＮＯＳ表达，并将其半定量与心肌病理变化程度进行相关分析，用比色法测定了血清中ＮＯ间接含量。结果 ①感染组小鼠心肌标本病理组织学检查均有不同

Expression of inducible nitric oxide synthase in the myocardium of viral myocarditis and the relationship between the expression levels and myocardial damages

Objective To study the expression of inducible nitric oxide synthase (iNOS) in the myocardium of viral myocarditis and the relationship between the expression levels and myocardial damages.Methods Twenty four BALB/C mice of 6 to 8 weeks were randomly divided into two groups:16 were injected intaperineally with CVB 3m and 8 served as control group.Immunohistochemistry technique was used to detect iNOS expression in the myocardium,and myocardial lesions were observed histopathologically.Furthermore,the relationship between iNOS expression levels and myocardlal damages were studied statistically by semi quantitative analysis.In addition,the serum nitrate contents were measured by using fluorospectrophotometry Results Histopathological study on the myocardium from infected mice revealed focal or diffuse lesions characterized by inflammatory cells and degeneration or necrosis of myocytes.The positive staining signals for iNOS proteins which mainly located in the infiltrating inflammatory cells and degenerative or necrotic myocytes were markedly detectable whereas there were no positive findings in the myocardium of control mice.The myocardial lesion score showed significantly positive correlation with the iNOS expression level (P<0 01) .The serum nitrate contents was significantly increased in infected mice as compared with the controls (P<0 01).Conclusion iNOS was expressed in the myocardium of viral myocar ditis murine model induced by CVB 3m .The large amounts of NO produced by iNOS may play an important role in the pathogenesis of viral myocarditis.