鼠疫耶尔森氏菌inv基因中IS1541的研究及应用

目的　了解鼠疫耶尔森氏菌 inv基因中 IS15 41的插入情况 ,为鼠疫耶尔森氏菌的鉴别诊断提供依据。方法　 PCR反应及 Southern杂交分析。结果　对来自不同疫源地的 5 0株鼠疫耶尔森氏菌的 inv基因中部分序列进行扩增 ,均可扩出一条长为 10 0 0 bp的片段 ,用 IS15 41作探针 ,对其进行 Southern杂交分析 ,杂交结果无差别 ,而对照菌株除假结核耶尔森氏菌扩出一条长约 30 0 bp左右的片段外 ,其它均为阴性。结论　鼠疫耶尔森氏菌 inv基因高度同源 ,其间均有 IS15 41插入 ;该方法有助于鼠疫耶尔森氏菌与其它菌株的鉴别诊断

Study on IS1541 in inv gene of Y.pestis

Objective To clarify the insertion of IS1541 in inv gene and supply help for identification of Y.pestis.Methods PCR amplification and southern blot analysis.Results The PCR amplification of inv gene was done on the 50 Y.pestis strains that isolated from different natural foci.One fragment of 1 000 bp was obtained in all tested strains,a IS1541 probe was applied on the product,the hybridization result is identical.One small frament was obtained in Y.pseudotuberculosis.There are no product in other control strains.Conclusions The inv genes are identical in all Y.pestis strains,IS1541 inserted to all of them;the approach will be helpful to the identification of Y.pestis.