系统性硬化症合并肺间质病变患者外周血中IL-8、CXCR1、CXCR2的表达及意义

目的分析系统性硬化症(SSc)合并肺间质病变(ILD)患者外周血中白介素8 (IL-8)、趋化因子受体1(CXCR1)、CXCR2表达的临床意义。方法选取2018年1月-2019年5月武汉市第五医院风湿免疫科治疗的系统性硬化症患者42例作为研究对象,其中合并ILD患者25例为SSc-ILD组,非ILD患者17例为SSc-非ILD组。另选取同期来院体检的健康者30例为健康对照组。检测3组受试者肺功能,外周血单个核细胞(PBMC)中CXCR1、CXCR2mRNA,血清IL-8、ESR、CRP、IgA、IgG、IgM、补体C3水平。采用Spearman进行检测指标的相关性分析。结果 SScILD组FEV1%、FEV1/FVC显著低于SSc-非ILD组和健康对照组(F/P=76.626/0.000,29.097/0.000),且SSc-非ILD组显著低于健康对照组(P<0.05);SSc-ILD组CXCR1、CXCR2 mRNA表达水平及血清IL-8、ESR、CRP、IgA、IgG、IgM水平显著高于SSc-非ILD组和健康对照组(F/P=581.600/0.000、401.103/0.000、149.551/0.000、34.990/0.000、37.207/0.000、189.508/0.000、117.657/0.000、12.708/0.000),且SSc-非ILD组各指标显著高于健康对照组(P<0.05);SSc患者外周血中CXCR1、CXCR2及血清IL-8水平与FEV1%呈显著负相关(r/P=-0.608/0.005、-0.538/0.048、-0.613/0.003),CXCR1、IL-8与FEV1/FVC呈显著负相关(r/P=-0.616/0.002、-0.581/0.0.15),CXCR2与FEV1/FVC无显著相关性(P>0.05);SSc合并ILD患者CXCR1、CXCR2、IL-8与CRP、ESR、IgA、IgG、IgM均呈正相关(P<0.05),与C3无显著相关性(P>0.05)。结论 IL-8与其受体CXCR1、CXCR2在系统性硬化症合并肺间质病变患者血清中均高表达,IL-8可能在SSc-ILD的发病机制中具有重要作用。

Expression and significance of IL-8,CXCR1 and CXCR2 in peripheral blood of patients with systemic sclerosis and pulmonary interstitial disease

Objective To analyze the clinical significance of the expression of interleukin-8(IL-8),chemokine receptor-1(CXCR1) and CXCR2 in peripheral blood of patients with systemic sclerosis(SSc) and pulmonary interstitial disease(ILD).Methods From January 2018 to may 2019,42 patients with systemic sclerosis were selected as the study object.Among them,25 patients with ILD were SSc-ILD group and 17 patients without ILD were SSc-non ILD group.Another 30 healthy people who came to the hospital for physical examination in the same period were selected as the control group.The lung function,CXCR1 and CXCR2 mRNA,serum IL-8,ESR,CRP,IgA,IgG,IgM and C3 levels in PBMC were measured.Spearman was used to analyze the correlation of detection indexes.Results The FEV1%,FEV1/FVC of the SSc-ILD group were significantly lower than those of the SSc non-ILD group and healthy control group(F/P=76.626/0.000,29.097/0.000),and the SSc non-ILD group was significantly lower than the healthy control group(P <0.05).The CXCR1,CXCR2 mRNA expression levels and IL-8,ESR,CRP,IgA,IgG,and IgM levels in the SSc-ILD group were significantly higher than those in the SSc non-ILD group and healthy controls(F/P=581.600/0.000,401.103/0.000,149.551/0.000,34.990/0.000,37.207/0.000,189.508/0.000,117.657/0.000,12.708/0.000),and the indexes of SSc non-ILD group were significantly higher than those of healthy control group(P<0.05).The levels of CXCR1,CXCR2 and serum IL-8 in patients with SSc were significantly negatively correlated with FEV1%(r/P=-0.608/0.005,-0.538/0.048,-0.613/0.003).CXCR1,IL-8 and FEV1/FVC were significantly negatively correlated(r/P=-0.616/0.002,-0.581/0.015).There was no significant correlation between CXCR2 and FEV1/FVC(P> 0.05).CXCR1,CXCR2,and IL-8 were positively correlated with CRP,ESR,IgA,IgG,and IgM in patients with SSc combined with ILD(P <0.05),and there was no significant correlation with C3(P>0.05).Conclusion IL-8,CXCR1 and CXCR2 are highly expressed in the serum of patients with systemic sclerosis and pulmonary interstitial disease.IL-8 may play an important role in the pathogenesis of SSc-ILD.