RPMI1640,DMEM及不同生长因子对大鼠颌下腺细胞生长作用的观察

目的　观察RPMI 16 4 0、DMEM及不同生长因子对大鼠颌下腺细胞增殖能力的影响 ,探讨最有利于大鼠颌下腺细胞的培养方法。方法　在RPMI 16 4 0或DMEM培养基中分别加入一定浓度的FBS(胎牛血清 )、EGF(表皮生长因子 )、HC(氢化可地松 )、INS(胰岛素 ) ,观察纤维细胞和腺细胞在不同培养基中的增殖能力。结果　在含5 %FBS、10ng/mlEGF、5 μg/mlHC、5 μg/mlINS的DMEM培养基中 ,上皮细胞生长良好 ,在只含血清的RPMI 16 4 0培养基中 ,纤维细胞生长旺盛 ,上皮样细胞生长不良 ,在不含血清的培养基中所有细胞均生长缓慢。结论　含有 5 %血清、10ng/mlEGF、5 μg/mlHC及 5 μg/mlINS的DMEM培养基最适合鼠涎腺上皮样细胞的生长

OBSERVATIONS OF EFFECTS OF 1640, DMEM AND DIFFERENT GROWTH FACTORS ON PRIMARY CULTURE OF MOUSE SUBMANDIBULAR GLAND

Objective To investigate the effect of 1640, DMEM and different growth factors on cultured mouse submandibular gland cells in order to acquire an optimal method for the culture of mouse submandibular gland. Methods Mouse submandibular gland cells were cultured in 1640, DMEM medium supplemented with different concentrations of serum, EGF, HC and INS. Results Epithelial cells cultured in DMEM grew better when supplemented with 5% FBS, 10ng/ml EGF, 5μg/ml HC and 5μg/ml INS. Conclusion DMEM medium supplemented with 5% FBS, 10ng/ml EGF, 5μg/ml HC and 5μg/ml INS was the optimal medium for the growth of cultured mouse submandibular gland cells.