| RP-HPLC法测定钩藤煎剂中钩藤碱含量及变化 |
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| 引用本文： | 刘清华,葛尔宁.RP-HPLC法测定钩藤煎剂中钩藤碱含量及变化[J].浙江中医药大学学报,2010,34(2):270-270,272. |
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| 作者姓名： | 刘清华 葛尔宁 |
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| 作者单位： | 浙江中医药大学,杭州,310053 |
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| 基金项目： | 浙江省教育厅资助课题 |
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| 摘 要： | 目的]测定钩藤中钩藤碱在沸水后下煎药法中煎出量及变化规律。方法]采用RP-HPLC法,HypersilBDSC18柱,(150mm×4.6mm,5μm),以甲醇∶水(70∶30)为流动相,流速1.0ml/min,波长254nm,柱温25℃。结果]钩藤碱在0.19μg～0.95μg范围内与峰面积线性关系良好r=0.9990。结论]钩藤在沸水后下煎药法中,其钩藤碱在汤药中的含量在11.4min达高峰,其后汤药中钩藤碱的含量逐渐降低。
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| 关 键 词： | 钩藤 钩藤碱 反相高效液相色谱法 |
RP-HPLC Method Measure the Contents and The Regularity of Rhychophyline in Ramulus Uncariae Cum Uncis |
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| Liu Qinghua,Ge Erning.RP-HPLC Method Measure the Contents and The Regularity of Rhychophyline in Ramulus Uncariae Cum Uncis[J].Journal of Zhejiang University of Traditional Chinese Medicine,2010,34(2):270-270,272. |
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| Authors: | Liu Qinghua Ge Erning |
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| Institution: | Zhejiang(University of Traditional Chinese Medicine,Hangzhou(310053),China ) |
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| Abstract: | Objective]To measure the contents of rhychophyline in uncaria,and its regularity of variety with decoction time.Methods]RP-HPLC method was used.The chromatographic conditions were listed below：Hypersil-BDS C18 column（150mm×4.6mm.5μm）,amobine phase composed of metranol-water（70∶30）,a flow rate of 1ml /min and wave lengths was 254nm,column temperature was 25 ℃.Results]In the range of 0.19μg ~0.95μg,the linear was very well between the content of rhychophyline and peak area.Conclusion]The contents of rhychophyline in decoction reached the peak at 11.4min after uncaria was added in boiling water. |
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| Keywords: | Ramulus Uncariae Cum Uncis Rhychophyline RP-HPLC |
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