2-氨基-3-苯基丙酰肼作为氨基脲敏感型胺氧化酶抑制剂的研究

目的：探讨人工合成2-氨基-3-苯基丙酰肼对氨基脲敏感型胺氧化酶（SSAO）的抑制效应。方法：根据文献合成2-氨基-3-苯基丙酰肼,通过核磁共振氢谱、红外光谱、质谱鉴定其结构。利用2-氨基-3-苯基丙酰肼对SSAO的抑制曲线,计算其IC50,结合透析实验确定2-氨基-3-苯基丙酰肼对SSAO抑制类型;建立高效液相色谱法测定2-氨基-3-苯基丙酰肼在水溶液中的浓度,验证其抑制类型。结果：2-氨基-3-苯基丙酰肼对SSAO的IC50为0.76μmol/L,透析后原液中2-氨基-3-苯基丙酰肼浓度为0.07μmol/L,其对SSAO的抑制率为78.0%。结论：2-氨基-3-苯基丙酰肼是SSAO不可逆抑制剂。

Study of 2-amino-3-phenylpropanehydrazide as Inhibitor of Semicarbazide Sensitive Amine Oxidase

Objective：To explore the artificial synthesis of 2-amino-3-phenylpropanehydrazide on semicarbazide sensitive amine oxidase（SSAO）inhibitory effect. Methods：2-amino-3-phenylpropanehydrazide was synthesized,and identified by IR,MS and 1H-NMR. According to the inhibition curve of 2-amino-3-phenylpropanehydrazide against SSAO,the IC50 was calculated. Dialysis experiment was performed to determine the inhibition type of 2- amino-3-phenylpropanehydrazide against SSAO. Concentration of 2-amino-3-phenylpropanehydrazide was analyzed by high performance liquid chromatography. Results：IC50 of 2-amino-3-phenylpropanehydrazide against SSAO was 0.76 μmol/L. The concentration of 2-amino-3-phenylpropanehydrazide in dialysis liquid after dialysis was 0.07 μmol/L,with inhibitory ratio of 78.0%. Conclusion：2-amino-3-phenylpropanehydrazide is an irreversible inhibitor of SSAO.