乳腺癌细针穿刺标本中端粒酶检测的诊断意义

目的 检测乳腺癌细针穿刺细胞标本中的端粒酶,探讨其在乳腺癌辅助诊断中的临床意义。方法 采用聚合酶链反应－端粒末端重复序列技术检测９９例乳腺细针穿刺标本（８３例乳腺癌、１２例乳腺良性病变和４例乳腺炎）中的端粒酶。

Significance of telomerase activity detection by fine-needle aspiration in patients with breast cancer

Objective To study the telomerase activity in samples of breast fine needle aspiration specimens and to investigate its potential clinical implications. Methods Ninety nine fine needle aspiration specimens from 83 patients with breast cancers, 12 with benign lesions and 4 with breast inflammation,were studied for the presence of telomerase activity by a PCR based telomere repeat amplification protocol (PCR TRAP). Results Among 83 fine needle aspiration specimens from patients with breast cancer, telomerase activity was detected in 61 of 69 cytologic positive specimens, 5 of 7 cytologic suspicious specimens and 4 of 7 cytologic negative specimens. Sensitivity of telomerase activity detection was 84.3% (70/83); the coincidence rate between telomerase detection and cytologic confirmation was 77.1%(64/83). Of 12 specimens from patients with benign breast lesions, 4 were positive for telomerase activity, but 4 specimens from patients with breast inflammation were all negative. The Combined use of cytological and telomerase detection can improve the detection rates from 83.1%(69/83) to 93.9%(78/83). Conclusion Detection of telomerase in breast tissue samples by fine needle aspiration is a sensitive and specific method, which implies that it would be helpful in preoperative cytological diagnosis of breast cancer.