| 车前子多糖对大鼠肝线粒体自由基防御功能的影响 |
| |
| 引用本文： | 袁从英,张然,车文文,张宁,冯娜,王素敏.车前子多糖对大鼠肝线粒体自由基防御功能的影响[J].中国老年学杂志,2011,31(4). |
| |
| 作者姓名： | 袁从英 张然 车文文 张宁 冯娜 王素敏 |
| |
| 作者单位： | 河北医科大学基础医学院药理教研室,河北,石家庄,050017 |
| |
| 基金项目： | 河北省自然基金项目(C2007000822); 河北省重点科研项目(20090314) |
| |
| 摘 要： | 目的探讨车前子多糖(PSP)对大鼠肝线粒体自由基防御功能的影响。方法差速离心法提取雄性SD大鼠肝线粒体,采用硫酸亚铁-维生素C(Fe2+-Vit C)激发剂,建立大鼠肝线粒体脂质过氧化(LPO)模型,比色法测定丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、过氧化氢酶(CAT)和抗氧化物酶(T-AOC)活性,观察PSP对各生化指标的影响。结果 PSP浓度在25、50和100 mg/L范围时,MDA含量分别为3.94±0.15,3.65±0.18和2.88±0.20,均显著低于模型组6.47±0.48(P<0.01),表明PSP对肝线粒体脂质过氧化有抑制作用,而对抗氧化物酶类活性均有激活作用,与模型组比较,均明显升高(P<0.01)。结论 PSP对大鼠肝线粒体自由基的防御功能可产生一定的影响,对治未病起到一定的防治作用。
|
| 关 键 词： | 硫酸亚铁-维生素C 车前子多糖 线粒体 抗自由基损伤 |
Effect of plantain seed polysaccharide on rat liver mitochondria free radicals defense functions |
| |
| YUAN Cong-Ying,ZHANG Ran,CHE Wen-Wen,et al..Effect of plantain seed polysaccharide on rat liver mitochondria free radicals defense functions[J].Chinese Journal of Gerontology,2011,31(4). |
| |
| Authors: | YUAN Cong-Ying ZHANG Ran CHE Wen-Wen |
| |
| Institution: | YUAN Cong-Ying,ZHANG Ran,CHE Wen-Wen,et al.Department of Pharmacology,Basic Medical College,Hebei Medical University,Shijiazhuang 050017,Hebei,China |
| |
| Abstract: | Objective To study influence of the plantain seed polysaccharide(PSP) on rats liver mitochondria free radicals defense functions.Methods Differential centrifugation method was used to extract male SD rat liver mitochondria,ferrous sulfate-vitamin C(Fe2+-VitC) activator was used to establish rat liver mitochondria lipid peroxidation(LPO) model,colorimetric was used to determine malondialdehyde(MDA),superoxide dismutase(SOD),glutathione peroxidase(GSH-Px),catalase(CAT) and antioxidant enzymes(T-AOC) activity ... |
| |
| Keywords: | Fe2 -VitC Plantain seed polysaccharide Mitochondria Anti-free radical damage |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
