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| 苯酚-硫酸法测定麻黄多糖含量研究 | |
| 引用本文： | 夏永刚,梁军,杨炳友,王秋红,匡海学. 苯酚-硫酸法测定麻黄多糖含量研究[J]. 中医药信息, 2011, 28(1): 33-35 |
| 作者姓名： | 夏永刚 梁军 杨炳友 王秋红 匡海学 |
| 作者单位： | 黑龙江中医药大学北药基础与应用研究省部共建教育部重点实验室,黑龙江省中药及天然药物药效物质基础研究重点实验室,黑龙江,哈尔滨,150040 |
| 基金项目： | 十二五国家科技部重大专项课题“重大新药创制”专项课题(2011ZX11102); 国家自然科学基金面上项目(30973870); 黑龙江省2009年研究生创新科研资金项目(YJSCX2009-171HLJ); 黑龙江中医药大学博士创新基金(2010) |
| 摘 要： | 目的：建立麻黄总多糖中多糖的含量测定方法。方法：采用苯酚-硫酸比色法。6％苯酚试剂1．0mL摇匀，迅速滴加浓硫酸5．0mL，即刻摇匀，放置5min后，置沸水浴加热15rain，取出冷却至室温（25℃），在490nrn波长处测定吸收度。结果：麻黄总多糖中多糖含量为66．43％，精密度及重复性良好，回收率为100．99％，RSD为1．91％。结论：此方法操作简便，准确性好，为麻黄总多糖中多糖含量测定提供了一个定量方法的同时为麻黄药材的质量控制提供了参考依据。 |
| 关 键 词： | 麻黄 多糖 苯酚-硫酸比色法 |
The Research on the Determination of Polysaccaride Contents of Ephedra by Phenol - sulfuric Acid Method |
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| XIA Yong-gang,LIANG Jun,YANG Bing-you,WANG Qiu-hong,KUANG Hai-xue. The Research on the Determination of Polysaccaride Contents of Ephedra by Phenol - sulfuric Acid Method[J]. Information on Traditional Chinese Medicine, 2011, 28(1): 33-35 | |
| Authors: | XIA Yong-gang LIANG Jun YANG Bing-you WANG Qiu-hong KUANG Hai-xue |
| Affiliation: | XIA Yong-gang,LIANG Jun,YANG Bing-you,WANG Qiu-hong,KUANG Hai-xue(Key Laboratory of Chinese Materia Medica(Heilongjiang University of Chinese Medicine,Ministry of Education,Heilongjiang key Laboratory of TCM Pharmacodynamic Material Bases,Harbin 150040,China) |
| Abstract: | Objective: To establish a UV method for the determination of polysaccarides in Ephedra sinica stapf.Method: The contents of polysaccharide were measured by phenol-sulfuric acid method.Result: The content of polysaccharide in Mahuang polysaccharide was 66.43%.Its recovery rate was 100.99%.RSD was 1.91%.Conclusion: This method is simple and accurate.It will provide an excellent quantitative method and reference for quality control of ephedra. |
| Keywords: | Ephedra Polysaccharide Phenol-sulfuric acid method |
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