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Comparative proteomics analysis of human gastric cancer |
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| Authors: | Li Wei Li Jian-Fang Qu Ying Chen Xue-Hua Qin Jian-Min Gu Qin-Long Yan Min Zhu Zheng-Gang Liu Bing-Ya |
| Affiliation: | 1. Putuo District Cancer Hospital,Shanghai University of Traditional Chinese Medicine,Shanghai 200025,China 2. Shanghai Key Laboratory of Diagnosis and Treatment for Gastrointestinal Cancer,Department of Surgery,Shanghai Institute of Digestive Surgery,Ruijin Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai 200025,China |
| Abstract: | AIM: To isolate and identify differentially expressed proteins between cancer and normal tissues of gastric cancer by two-dimensional electrophoresis (2-DE) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS).METHODS: Soluble fraction proteins of gastric cancer tissues and paired normal tissues were separated by 2-DE.The differentially expressed proteins were selected and identified by MALDI-TOF-MS and database search.RESULTS: 2-DE profiles with high resolution and reproducibility were obtained.Twenty-three protein spots were excised from sliver staining gel and digested in gel by trypsin,in which fifteen protein spots were identified successfully.Among the identified proteins,there were ten over-expressed and five under-expressed proteins in stomach cancer tissues compared with normal tissues.CONCLUSION: In this study,the well-resolved,reproducible 2-DE patterns of human gastric cancer tissue and paired normal tissue were established and optimized and certain differentially-expressed proteins were identified.The combined use of 2-DE and MS provides an effective approach to screen for potential tumor markers. |
| Keywords: | Proteome Two-dimensional polyacrylamide gel electrophoresis Mass spectrometry Gastric cancer Tumor markers |
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