磁性四氧化三铁纳米颗粒作用于前成骨细胞的生物相容性

Biocompatibility of magnetic ferrosoferric oxide nanoparticles in preosteoblasts

BACKGROUND: Investigations on toxic mechanism and safety of magnetic ferrosoferric oxide (Fe3O4) nanoparticles are extremely necessary when these nanoparticles as an emerging material for bone tissue engineering are implanted into the living body.OBJECTIVE: To investigate the biocompatibility of magnetic Fe3O4 nanoparticles with preosteoblasts.METHODS: Mouse preosteoblasts were cultured in 0, 200, 400, 800 mg/L magnetic Fe3O4 nanoparticles. After 24 hours, alkaline phosphatase activity, osteocalcin level, cell proliferation rate, cellular morphology, cytoskeleton variation, cell apoptosis and autophagy-related genes, such as Caspase-3, LC3A, LC3B, were detected by alkaline phosphatase assay kit, ELISA kit, cell counting kit-8 kit, inverted microscope, laser confocal microscopy and real-time PCR, respectively. RESULTS AND CONCLUSION: After 24 hours of culture, there ware no significant differences between 200 mg/L group and control group. However, in the groups of 400 and 800 mg/L, the ratio of alkaline phosphatase activity to total protein and osteocalcin level increased, the cell proliferation rate decreased, cellular morphology and cytoskeleton changed remarkably, LC3B expression was up-regulated compared with the control group. Additionally, there were also no significant differences in the expression of Caspase-3 and LC3A between 400 and 800 mg/L groups and control group. Therefore, magnetic Fe3O4 nanoparticles at high level contributes to cytotoxicity and up-regulation of LC3B expression, and affects cellular morphology, cytoskeleton and cell proliferation rate, although these nanoparticles can increase the osteoblastic differentiation.