脂肪间充质干细胞移植后肝硬化大鼠血生化指标的变化

Variation of blood biochemical indices in liver cirrhosis rats after adipose-derived mesenchymal stem cell transplantation

BACKGROUND:Stem cell transplantation is a promising treatment of advanced liver disease, and adipose-derived mesenchymal stem cells have become another kind of popular cells following bone marrow mesenchymal stem cells.OBJECTIVE:To investigate the influence of adipose-derived mesenchymal stem cell transplantation on blood biochemical indices of liver cirrhosis rats. METHODS:Sixty rats were equally randomized into normal control, model and cell transplantation groups. Model rats of liver cirrhosis were made in the latter two groups through intragastric administration of carbon tetrachloride. One week after successful modeling, rats were given intraperitoneal injection of adipose-derived mesenchymal stem cell suspension in the cell transplantation group, and given normal saline in the other two groups.RESULTS AND CONCLUSION:Compared with the normal control group, the model group showed a significant increase in the levels of alanine aminotransferase, aspartate aminotransferase, total bilirubin, total protein in liver tissues, serum level of malondialdehyde, 15-minute indocyanine green retention rate and degree of hepatic fibrosis, and a significant decrease in serum albumin level, serum albumin/globulin, levels of glutathione peroxidase and cyclic guanosine monophosphate in liver tissues. On the contrary, these indicators were all improved in the cell transplantation group compared with the model group. Moreover, CM-Dil-positive cells were visible in the liver tissue of rats undergoing adipose-derived mesenchymal stem cell transplantation. All these findings indicate that adipose-derived mesenchymal stem cell transplantation can reduce liver cirrhosis in rats by acting on blood biochemistry levels.