体外构建3D人工皮肤

BACKGROUND: Epidermis is unable to differentiate into stratum corneum and other parts in the previous artificial skin constructed using three-dimensional printing.OBJECTIVE: To optimize the method of artificial skin construction to obtain more feasible artificial skin. METHODS: Type I collagen extracted from rat-tail and bovine tail was purified. Primary dermal fibroblasts and keratinocytes were isolated from children’s foreskin, and then embedded in bovine or rat tail collage to construct the dermis; keratinocytes were seeded on the dermis for 7 days, followed by 7-day air liquid interface, and the artificial skin was finally achieved. The contraction and hydrophobicity by water contact angle were detected, as well as the morphology was observed by hematoxylin-eosin staining, sirius red staining and immunofluorescence analysis.RESULTS AND CONCLUSION: Seven days after fibroblasts embedded in collagen, the area of bovine collagen was reduced by 21%, while that of rat tail collagen decreased about a half. The water contact angle of the skin constructed by bovine tail collagen was similar to that of human skin, which was much higher than that of the skin constructed by rat tail collagen. Sirius red staining found that the skin constructed by bovine tail collagen had stronger refractivity, more intact structure and thicker fibers than those of the skin constructed by rat tail collagen. Moreover, more multilayer keratinocytes appeared in the skin constructed by bovine tail collagen through hematoxylin-eosin staining. In conclusion, bovine tail collagen is more available for the artificial skin construction.中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Artificial skin preparation using three-dimensional printing in vitro