力生长因子促进骨髓间充质干细胞向成骨细胞的分化

Mechano growth factor promotes the differentiation of bone marrow mesenchymal stem cells into osteoblasts

BACKGROUND:Mechano growth factor has the potential to activate muscle satellite cells and promote myogenic cell growth, and has dual roles in maintaining bone mass and repairing bone defects.OBJECTIVE:To explore the mechanism underlying osteogenic differentiation of rabbit bone marrow mesenchymal stem cells promoted by the mechano growth factor.METHODS:The best concentration and time of mechano growth factor to promote osteogenic differentiation of rabbit bone marrow mesenchymal stem cells were detected by MTT. The mRNA and protein expressions of alkaline phosphatase and osteocalcin were detected by qPCR and western blot, respectively. The phosphorylation level of AKT and mTOR were detected by western blot assay. RESULTS AND CONCLUSION:The best concentration and time of mechano growth factor was 45 μg/L and 5 days for promoting the osteogenic differentiation of rabbit bone marrow mesenchymal stem cells. The expressions of alkaline phosphatase and osteocalcin at mRNA and protein levels were highest after 4-hour intervention with 45 μg/L mechano growth factor, and meanwhile, the phosphorylation levels of mTOR and AKT were also highest. These findings indicate that the mechano growth factor can promote the differentiation of rabbit bone marrow mesenchymal stem cells into osteoblasts via PI3K/AKT pathway, and its best concentration and time are 45 μg/L and 4 hours, respectively.