药物和行为干预对缺血缺氧性脑损伤大鼠功能恢复的影响

目的探讨联合应用神经营养药物及行为干预对缺氧缺血性脑损伤后大鼠神经功能的影响。方法建立早产缺血缺氧性脑损伤的大鼠模型后随机分为GM1治疗组、NGF治疗组、药物联合(GM1+NGF)治疗组、行为干预治疗组、药物联合+行为干预治疗组、模型对照组,每组8只。另取7只入假手术组。分别给予腹腔注射单唾液酸四己糖神经节苷脂钠(GM1)、鼠神经生长因子(NGF)或(和)行为干预治疗,待30日龄时进行悬吊试验和斜坡试验。结果析因设计分析,悬吊试验中,GM1的主效应有统计学意义(P<0.05),NGF的主效应没有统计学意义(P>0.05);在斜坡试验中两者主效应均有统计学意义(P<0.05);两者在悬吊试验和斜坡试验中都没有交互效应(P>0.05)。药物治疗与行为干预在悬吊试验和斜坡试验中主效应都有统计学意义(P<0.05),且存在着交互效应(P<0.05)。结论 GM1能改善实验动物的肌力和随意运动以及躯体平衡和协调功能;NGF能改善躯体平衡和协调功能。两者联用无明显优势。行为干预能够提高实验动物的肌力和随意运动能力,改善实验动物的躯体协调及平衡功能,联合药物治疗可取得协同效应。

Effects of Neurotrophics and Rehabilitation on Neural Function after Hypoxic-ischemic Brain Injury in Rats

Objective To investigate the effects of neurotrophics and rehabilitation on the neural function recovery after the hypoxic-ischemic brain injury in rats.Methods Model rats of hypoxic-ischemic brain injury were given monosialotetrahexosylganglioside sodium(GM1,n=8),mouse nerve growth factor(NGF,n=8),both drugs(n=8),rehabilitation(n=8),both drugs+rehabilitation(n=8) and control(no intervention,n=8).The other 7 rats was as sham group.They were assessed with hanging and inclined plane test when 30 days old.Results In the factorial design,main effect of GM1 was significant(P<0.05) but NGF(P>0.05) in hanging test;while both were significant in inclined plane test.The interaction was not significant(P>0.05) in both tests.The main effects of drugs and rehabilitation were significant(P<0.05),as well as the interaction(P<0.05) in both tests.Conclusion GM1 may significantly improve muscle strength,voluntary movement,balance and coordination functions,and NGF may improve the balance and coordination functions.No interaction is found when combined with the two therapies.Rehabilitation may significantly improve muscle strength,voluntary movement,coordination and balance,and synergy effects can be gained when combined with the drugs.