注射硫酸软骨素酶对大鼠脊髓损伤后腓肠肌乙酰胆碱酯酶的变化及运动功能的影响

目的探讨注射硫酸软骨素酶ABC对成年大鼠脊髓损伤后不同时期后肢运动功能的恢复及腓肠肌运动终板内乙酰胆碱酯酶的影响。方法 10周龄Wistar雄性大鼠40只,采用脊髓半横断法制作模型,健侧作为对照组(A组),患侧随机分为单纯脊髓损伤组(B组)及术后注射硫酸软骨素酶ABC组(C组)。术后采用BBB评分法进行行为学观察,分别于损伤后3 d、7 d、14 d和28d各选取5只大鼠,酶化学染色法检测腓肠肌中乙酰胆碱酯酶(AChE)的表达。结果 C组在术后14~28 d BBB评分高于B组(P<0.05);B组和C组与A组相比,腓肠肌AChE活性均降低,但C组于术后14~28 d AchE活性高于B组(P<0.05)。结论大鼠脊髓损伤后注射硫酸软骨素酶可以提高AChE的活性,并可提高大鼠患肢的运动功能。

Effects of Chondroitinase ABC Injection on Motor and Acetylcholinestarase in Gastrocnemius of Spinal Cord Injuried Adult Rats

Objective To investigate the effects of chondroitinase ABC injection on motor and acetylcholinestarase(AchE) expressed in the motor end-plate of gastrocnemius in adult rats with spinal cord injury.Methods The spinal cords in T10 of 40 male Wistar rats(10 weeks old) were exposed and the right sites were semi-transected.The left sites were considered as the control(group A),the transected sites were as the injured group(group B) and chondroitinase ABC-treated group(group C).The rats were assessed with Basso,Beattie & Bresnahan(BBB) score for ethological test,and sacrificed 3 d,7 d,14 d and 28 d after operation.The AchE expressed in gastrocnemius was detected with zymochemistry staining.Results BBB score in group C was significant higher than that in group B 14~28 d after operation,while the AchE activity in group B and C was lower than that in group A(P<0.05),and lower in group B than in group C(P<0.05).Conclusion Chondroitinase ABC injection can enhance AchE activity and promote the locomotor recovery after spinal cord injury in adult rats.