| 表皮生长因子受体对鼻咽癌放疗后细胞增殖的影响 |
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| 引用本文： | 卜俊国,张积仁. 表皮生长因子受体对鼻咽癌放疗后细胞增殖的影响[J]. 海南医学院学报, 2012, 18(9): 1194-1195,1199 |
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| 作者姓名： | 卜俊国 张积仁 |
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| 作者单位： | 南方医科大学珠江医院肿瘤中心,广东广州,510282 |
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| 基金项目： | 广东省自然科学基金项目(8451051501000519)~~ |
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| 摘 要： | 目的:观察表皮生长因子受体(epidermal growth factor receptor,EGFR)对射线照射后的鼻咽癌细胞的增殖的影响。方法:用shRNA-EGFR转染人鼻咽癌细胞系CNE1,应用X射线照射转染EGFRSiRNA前后的细胞,MTT实验比较转染干扰质粒前后照射后细胞增殖变化。结果:shRNA-EGFR成功转染CNE1细胞。CNE1细胞干扰后照射较干扰前照射增殖减低。结论:通过抑制鼻咽癌细胞EGFR的表达,可以抑制鼻咽癌放疗后的增殖。
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| 关 键 词： | 鼻咽肿瘤 表皮生长因子受体 细胞系 辐射 增殖 |
Effect of EGFR on proliferation of nasopharyngeal carcinoma cell after chemotherapy |
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| BU Jun-guo , ZHANG Ji-ren. Effect of EGFR on proliferation of nasopharyngeal carcinoma cell after chemotherapy[J]. Journal of Hainan Medical College, 2012, 18(9): 1194-1195,1199 |
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| Authors: | BU Jun-guo ZHANG Ji-ren |
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| Affiliation: | (Oncology Center,Zhujiang Hospital,Southern Medical University,Guangzhou 510282,China) |
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| Abstract: | Objective: To investigate the effect of EGFR on proliferation of human nasopharyngeal carcinoma cell after chemotherapy.Methods:shRNA-EGFR as well as various controls was transfected into CNE1,human nasopharyngeal carcinoma cell line.The proliferation of cells after chemotherapy was detected by MTT experiment,and was compared before and after interfering.Results:shRNA-EGFR could be effectively delivered into nasopharyngeal carcinoma cells.Proliferation of CNE1 irradiated cells was significantly decreased after interference.Conclusions:The proliferation of nasopharyngeal carcinoma can be inhibited through silencing EGFR. |
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| Keywords: | Epidermal growth factor receptor Nasopharyngeal carcinoma Cell line Radiation Proliferation |
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