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Isolation and characterization of novel microsatellite markers from the leafhopper Hishimonus phycitis distant (Hemiptera: Cicadellidae) |
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| Authors: | Mahnaz Shabani Mohsen Mardi Alimorad Sarafrazi Sepehr Mohajeri Naraghi Heshmatollah Rahimian Mahmoud Shojaee Mehrshad Zeinolabedini |
| Affiliation: | 1. Department of Genomics, Agricultural Biotechnology Research Institute of Iran (ABRII), Mahdasht Road, P. O. Box: 31535-1897, Karaj, Iran 4. Department of Entomology, Faculty of Agriculture and Natural Resources, Islamic Azad University, Science and Research Branch, Tehran, Iran 2. Department of Insect Taxonomy Research, Iranian Research Institute of Plant Protection, Tehran, Iran 3. Department of Plant Pathology, Agricultural Sciences and Natural Resources University of Sari, Sari, Iran |
| Abstract: | The leafhopper Hishimonus phycitis has received considerable attention as a vector of witches’ broom disease of lime phytoplasma. In the present study, nine polymorphic microsatellite loci were isolated from a repeat-enriched genomic library of H. phycitis. The number of alleles varied between 2 and 4 with an average of 2.80 per locus. The observed and expected heterozygosities ranged from 0.28 to 0.84 and 0.28 to 0.80, respectively. The Polymorphism Information Content varied from 0.25 to 0.75 with an average of 0.55. These microsatellites will be useful for future genetic studies of H. phycitis populations and could help in the development of an efficient control strategy against this vector. |
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