18氟-脱氧葡萄糖诱导Lewis肺癌细胞凋亡的实验研究

目的：观察不同浓度18氟-脱氧葡萄糖（18F-FDG）对Lewis肺癌细胞凋亡的影响,并探讨其作用机制。方法：指数生长期Lewis肺癌细胞分为18F-FDG实验组（为92.5×104Bq/ml组、185×104Bq/ml组、370×104Bq/ml组和740×104Bq/ml组）和对照组,应用光镜、电镜、流式细胞术（flow cytometry,FCM）和免疫组化技术检测不同浓度18F-FDG作用于体外培养的Lewis肺癌细胞后,诱导脂质氧化、细胞凋亡及bcl-2和bax基因表达情况。结果：18F-FDG能诱导Lewis肺癌细胞产生凋亡形态学变化,随着18F-FDG浓度增加,脂质氧化增强、细胞凋亡率增加、bcl-2表达减弱、bax表达增强（P〈0.01）。结论：18F-FDG能诱导Lewis肺癌细胞凋亡,且凋亡具有剂量依赖性。

Lewis lung cancer cell apoptosis induced by 18F-fluorodeoxyglucose

Objective：To investigate the influence of 18F-fluorodeoxyglucose（18F-FDG） on the apoptosis of Lewis lung cancer cell line and elucidate its possible mechanism.Methods：Lewis lung cancer cells were conventionally cultured.Cells during the stage of logarithmic growth were divided into 18F-FDG treatment group and control group.Using light microscopy,trammissional electron microscopy,flow cytometry and immunohistochemical techniques to analyse apoptosis induced by different concentrations of 18F-FDG and expressions of bcl-2 and bax genes.Results：Lewis lung cancer cell showed morphological and physiologic chemistry changes of apoptosis after exposure to 18F-FDG.Lipid peroxidation and apoptosis rate increased with the increase of the concentration of 18F-FDG.The expression of bcl-2 gene decreased and that of bax gene increased.Conclusions：18F-FDG may induce apoptosis of Lewis lung cancer cells,and apoptosis rate increased with the increase of the concentration of 18F-FDG.