微波消解/ICP-MS法测定胎儿肾脏和小脑中稀土元素的研究

目的　测定深圳市正常中期引产胎儿肾脏和小脑中多种稀土元素的含量 ,为研究人类生命不同阶段稀土元素水平提供基础资料。方法　样品经HNO3+H2 O2 微波消解系统消解后 ,用电感耦合等离子质谱(ICP MS)检测胎儿肾脏和小脑中La、Ce、Pr、Nd、Sm、Eu、Gd、Tb、Dy、Ho、Er、Tm、Yb、Lu 14种稀土元素。结果　所检测的 14种稀土元素 ,La在肾脏中的含量最高为 0 4 2 0 μg kg,Ce在小脑中的含量最高为 0 .36 3μg kg,Yb在肾脏和小脑中的含量均最低 ,分别为 0 . 10 3μg kg和 0. 0 98μg kg。在胎儿肾脏和小脑中 ,轻稀土元素 (La～Sm)的总含量分别为 1 .4 2 7μg kg、1. 2 36 μg kg ,中稀土元素 (Eu～Gd)的总含量分别为 0 . 4 6 9μg kg、0 .4 32 μg kg ,重稀土元素 (Tb～Lu)的总含量分别为 1 .387μg kg、1 .4 18μg kg。 结论　上述 14种稀土元素均可在胎儿肾脏和小脑中检出 ,并具有一定的分布特点。

Determination of rare earth elements in fetal kidney and cerebella by microwave dissolution and inductively coupled plasma-MS spectrometry

OBJECTIVE: Study the contents of rare earth elements in normal fetal kidney and cerebella, and provide base data for the levels of rare earth elements in different phase of human development. METHODS: The samples were digested by closed-vessel microwave HNO3 + H2O2 dissolution system and directly tested by ICP-MS for the contents of La, Ce, Pr, Nd, Sm, Eu, Gd, Tb, Dy, Ho, Er, Tm, Yb and Lu in normal fetal kidney and cerebella. RESULTS: La mainly existed in fetal kidney (0.420 microg/kg), Ce mainly existed in fetal cerebella(0.363 microg/kg). Yb was little in both kidney and cerebella (0.103 microg/kg and 0.098 microg/kg). The contents of light rare earth elements in fetal kidney and cerebella were 1.427 microg/kg and 1.236 microg/kg, the contents of medium rare earth elements in fetal kidney and cerebella were 0.469 microg/kg and 0.432 microg/kg, and the contents of heavy rare earth elements in fetal kidney and cerebella were 1.387 microg/kg and 1.418 microg/kg. CONCLUSION: The rare earth elements all can be tested in fetal kidney and cerebella.