A standardized procedure for serum protein electrophoresis on cellulose acetate membrane strips |
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| Affiliation: | 1. Federal University of São Carlos, Department of Physiotherapy, Rod Washington Luis Km 235, São Carlos 13565-905, Brazil;2. Federal University of São Paulo, Department of Bioscience, Av. Ana Costa 95, Santos 11050-240, Brazil;3. Federal University of São Carlos, Department of Genetics and Evolution, Rod Washington Luis Km 235, São Carlos 13565-905, Brazil;4. University of São Paulo, School of Pharmaceutical Sciences of Ribeirão Preto, Department of Clinical Analysis, Toxicological and Bromatological, Av. do Café 95, Ribeirão Preto, Brazil, 14049-900, Brazil;5. State University of Campinas, Department of Genetics and Evolution, Cidade Universitária Zeferino Vaz, Campinas 13083-970, Brazil;6. Brazilian National Center for Research in Energy and Materials, Brazilian Biosciences National Laboratory, Giuseppe Máximo Scolfaro 10.000, Campinas 13083-970, Brazil |
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| Abstract: | Detailed directions for serum protein electrophoresis on cellulose acetate strips using the EEL electrophoresis apparatus with adapter cells, for the staining and quantitative determinations of the relative values of the fractions have been presented. Some problems inherent in the methods and apparatus, as well as in the calculation of results, are discussed. Standard values for the relative values of serum proteins in terms of the total protein and in terms of the total globulins are presented, with statistical analyses. These values are not to be construed as universally correct, nor useful in those laboratories using different methods and equipment, but are only applicable clinically when the apparatus and the methods described herein are used as directed. |
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