| 骨髓间充质干细胞在梗死心肌移植再生后nesprin蛋白表达的实验研究 |
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| 引用本文： | 杨文钢,薛松,汪铮,连锋,徐根兴,刘沙,黄日太,朱洪生,张谷兰.骨髓间充质干细胞在梗死心肌移植再生后nesprin蛋白表达的实验研究[J].江苏大学学报(医学版),2010,20(2):148-152,I0002. |
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| 作者姓名： | 杨文钢 薛松 汪铮 连锋 徐根兴 刘沙 黄日太 朱洪生 张谷兰 |
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| 作者单位： | 1. 上海交通大学医学院附属仁济医院心胸外科,上海,200127
2. 上海交通大学医学院附属仁济医院消化疾病研究所,上海,200127 |
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| 基金项目： | 上海市科委基金资助项目 |
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| 摘 要： | 目的:研究骨髓间充质干细胞(mesenchymal stem cells,MSCs)移植到急性心肌梗死区心肌样细胞再生后nesprin蛋白的表达情况.方法:大鼠16只,随机分为实验组和对照组,每组8只.利用密度梯度分离法和贴壁培养MSCs,流式细胞仪检测MSCs表面相关抗原、DAPI标记MSCs备用;SD大鼠开胸手术结扎心脏冠脉左前降支建立心肌梗死模型,并在实验组梗死区注射MSCs,对照组注射DMEM;术后3周取梗死区心肌,利用免疫细胞学化学方法鉴定肌节肌动蛋白和肌钙蛋白的表达,免疫荧光技术和蛋白质印迹鉴定nesprin蛋白在MSCs移植后的表达.结果:MSCs移植到梗死缺血区3周后可以用DAPI示踪、并有心肌细胞特异蛋白呈阳性表达;免疫荧光和蛋白质印迹鉴定在MSCs移植前后有nesprin蛋白的表达,移植后出现nesprin蛋白表达量的增加.结论:MSCs在梗死心肌内移植具有分化再生的能力,nesprin蛋白在MSCs移植后出现表达量的增加,可能在MSCs分化为心肌细胞样过程中起到一定的作用.
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| 关 键 词： | nesprin蛋白 间充质干细胞 细胞移植 心肌细胞再生 |
Study on the regeneration of transplanted mesenchymal stem cells in the infarcted myocardium and identify the expression of nesprin protein |
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| YANG Wen-gang,XUE Song,WANG Zheng,LIAN Feng,XU Gen-xing,LIU Sha,HUANG Ri-tai,ZHU Hong-sheng,ZHANG Gu-lan.Study on the regeneration of transplanted mesenchymal stem cells in the infarcted myocardium and identify the expression of nesprin protein[J].Journal of Jiangsu University Medicine Edition,2010,20(2):148-152,I0002. |
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| Authors: | YANG Wen-gang XUE Song WANG Zheng LIAN Feng XU Gen-xing LIU Sha HUANG Ri-tai ZHU Hong-sheng ZHANG Gu-lan |
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| Institution: | YANG Wen-gang1,XUE Song1,WANG Zheng2,LIAN Feng1,XU Gen-xing1,LIU Sha1,HUANG Ri-tai1,ZHU Hong-sheng1,ZHANG Gu-lan1(1.Department of Cardiothoracic,2.Institute of Digestive Diseases,Renji Hospital Affiliated to Medical College of Shanghai Jiaotong University,Shanghai 200127,China) |
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| Abstract: | Objective:Identify the differentiation of rat bone mesenchymal stem cells(MSCs)into myocardium-like cells after MSCs was transplanted into acute myocardial infarction.Then,identify the expression of nesprin protein.Methods:Sixteen SD rats were randomly divided into experimental group (n=8) and the control group (n=8).MSCs were isolated and purified from the bone marrow of rats by density gradient centrifugation and adhering to the culture plastics.Surface-associated antigens of MSCs were detected by flow cy... |
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| Keywords: | nesprin protein mesenchymal stem cells cell transplantation cardiomyocyte regeneration |
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