| 干扰素α和白细胞介素2联合激活的白血病骨髓对K562细胞的杀… |
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| 引用本文： | 李成文,严文伟.干扰素α和白细胞介素2联合激活的白血病骨髓对K562细胞的杀…[J].中华血液学杂志,1997,18(8):410-412. |
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| 作者姓名： | 李成文 严文伟 |
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| 摘 要： | 评价于干扰素α和白细胞介素２联合激活的白血病缓解期骨髓对白血病细胞的杀伤作用。方法：半固体集浇培养法。结果：ＩＵＬ－２激活３天的白血病缓解髓对Ｋ５６２细胞有杀伤作用，杀伤对数级达０．１３－２．３０。ＩＮＦα可明显增强ＩＬ－２激活骨髓的杀伤作用，其对Ｋ５６２细胞的杀伤率可进一步提高，达０．３０〉３．１５对数级，ＩＬ－２和ＩＮＦα联合对骨髓ＣＦＵ－ＧＭ和Ｋ５６２细胞无明显抑制作用，小剂量ＩＬ－２与ＩＦ
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| 关 键 词： | 白细胞介素2 干扰素 白血病 K562细胞 |
The killing effect of IL-2 and IFN-alpha activated bone marrow on K562 leukemic cell] |
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| C Li,W Yan,Y Hao,S Feng,L Qiu,H Liu,J Han,X Li,S Feng.The killing effect of IL-2 and IFN-alpha activated bone marrow on K562 leukemic cell][J].Chinese Journal of Hematology,1997,18(8):410-412. |
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| Authors: | C Li W Yan Y Hao S Feng L Qiu H Liu J Han X Li S Feng |
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| Institution: | Institute of Hematology and Blood Diseases Hospital, CAMS and PUMC, Tianjin 300020. |
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| Abstract: | OBJECTIVE: To evaluate the killing effect of IL-2 and IFN-alpha activated bone marrow cells on K562 cells. METHODS: Semi-solid colony assay was used. RESULTS: Bone marrow from leukemia patients in remission was activated in vitro with IL-2 for 3 days. The activated bone marrow (ABM) displayed killing effects of 0.31 approximately 2.30 logs on K562 cells, this killing effects was further increased to 0. 30 approximately > 3.15 logs when IFN-alpha added with IL-2 to the marrow for activation. IL-2 alone or in combination with IFN-alpha showed no inhibition of CFU-GM and K562 cells. Compared with IL-2 or IFN-alpha alone, the combination of the two cytokines could more effectively maintain the killing effect of ABM on leukemic cells. CONCLUSION: IFN-alpha can augment the purging effect of IL-2 ABM and combination of the two cytokines can effectively maintain the cytotoxicity of ABM. |
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