| 纳米粒沉淀法制备阳离子载基因PLA-PEG纳米粒 |
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| 引用本文： | 邹伟伟,张娜. 纳米粒沉淀法制备阳离子载基因PLA-PEG纳米粒[J]. 中国生化药物杂志, 2009, 30(1) |
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| 作者姓名： | 邹伟伟 张娜 |
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| 作者单位： | 山东大学,药学院,药物制剂研究所,山东,济南,250012 |
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| 摘 要： | 目的建立纳米粒沉淀法制备阳离子PLA-PEG纳米粒的方法。方法采用单因素设计考察不同影响因素对纳米粒粒径大小的影响,在单因素考察的基础上采用正交设计优化处方,制备了粒径较小,正电荷适中的阳离子PLA-PEG纳米粒。并对纳米粒的物理性质如物理形态,平均粒径,粒度分布,Zeta电位,DNA结合率,体外细胞转染能力等进行了考察。结果采用纳米粒沉淀法,通过优化处方和工艺制得的纳米粒外观圆整,呈类球形,大小均匀,平均粒径为89.7 nm,粒径分布指数为0.185,表面荷较高的正电荷,Zeta电位为+28.9 mV,能够高效的结合DNA且能够成功的转染Hela细胞。结论优化确定了纳米粒沉淀法制备阳离子PLA-PEG纳米粒的处方和工艺,可以制备满足细胞转染要求的阳离子载基因纳米粒。
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| 关 键 词： | 纳米粒沉淀法 PLA-PEG纳米粒 非病毒基因载体 制备 |
Preparation of cationic gene loaded PLA-PEG nanoparticles by nanoprecipitation method |
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| ZOU Wei-wei,ZHANG Na. Preparation of cationic gene loaded PLA-PEG nanoparticles by nanoprecipitation method[J]. Chinese Journal of Biochemical Pharmaceutics, 2009, 30(1) |
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| Authors: | ZOU Wei-wei ZHANG Na |
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| Affiliation: | Institute of Pharmaceutics;School of Pharmaceutical Science;Shandong University;Jinan 250012;China |
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| Abstract: | Purpose To develop a nanoprecipitation method for preparation of cationic PLA-PEG nanoparticles(PLA-PEG-NPs).Methods The effects of different formulations and technical factors on the particle size of nanoparticles were investigated by single factor investigation.Based on single factor investigation,the formulation was optimized by orthogonal design.The optimal nanoparticles were characterized in terms of morphology,average particle size,polydispersion index(PDI),Zeta potential,DNA binding efficiency and in... |
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| Keywords: | nanoprecipitation method PLA-PEG nanoparticles non-viral gene vector preparation |
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