冰水灌胃应激建立便秘表现肠功能紊乱大鼠模型

背景:应激是引起肠功能紊乱的重要原因之一。建立适当的动物模型对于研究肠功能紊乱性疾病的病因和发病机制至关重要。目的:通过冰水灌胃应激建立便秘表现肠功能紊乱大鼠模型。方法:36只Sprague-Dawley大鼠随机分为6组,A1、A2组和B1、B2组分别予冰水和常温水灌胃14 d,C1、C2组不予灌胃,作为正常对照。A2、B2、C2组于灌胃结束后次日行小肠推进实验,A1、B1、C1组于灌胃期间和其后的14 d每日计数24 h粪便粒数,称量粪便质量,结束后次日行小肠推进实验。结果:冰水灌胃组第2周末24 h粪便粒数、质量和小肠墨汁推进率显著低于常温水灌胃组和正常对照组(P0.05),并保持至第4周末,组内第2周末与第4周末间差异无统计学意义。结论:以冰水灌胃应激建立的便秘表现肠功能紊乱大鼠模型能模拟人类肠功能紊乱且重复性、稳定性好,为相关疾病的研究提供了条件。

Establishment of Rat Model with Constipation-predominant Bowel Dysfunction by Intragastric Perfusion of Ice Water

Background： Stress is one of the major factors involved in the development of bowel dysfunction. It is important to construct an ideal animal model for studying the etiological factors and pathogenic mechanisms of bowel dysfunction. Aims： To construct a rat model with constipation-predominant bowel dysfunction by intragastric perfusion of ice water. Methods： Thirty-six Sprague-Dawley rats were randomly allocated into six groups, four groups were perfused intragastrically with ice water （group A1, A2） or room temperature water （group B1, B2） for 14 days, and two groups without any intervention were served as controls （group C1, C2）. The 24-hour fecal granules number and stool weight in group A1, B1 and C1 were measured daily in the course of 14-day intragastric perfusion and the following 14 days. Small bowel propulsion test was performed in group A2, B2 and C2 on the 15th day, and in group A1, B1 and C1 on the 29th day, respectively. Results： The 24-hour fecal granules number and stool weight, as well as the small bowel ink propulsion rate at the end of the 2nd week were significantly decreased in rats perfused with ice water when compared with rats perfused with room temperature water and the controls （P〈0.05）. These changes were sustained till the end of the 4th week, and no significant differences were found between these two time points. Conclusions： Rat model with constipation-predominant bowel dysfunction induced by intragastric perfusion of ice water can mimic the disease seen in human beings with reproducibility and stability. It may provide a favorable model for the study of bowel dysfunction.