阴虚对荷瘤小鼠肿瘤生长的影响

目的为阐明阴阳失衡对肿瘤生长的影响。方法采用甲状腺素和利血平、氢化可的松分别制作阴虚、阳虚模型,并接种S180瘤株;用RT-PCR的方法分析肿瘤细胞基因表达的水平。结果阴虚组的瘤重均高于正常和阳虚组,而差异不明显(P>0.05),但影响肿瘤增殖的PCNA基因表达水平明显高于正常和阳虚组(P<0.05)。结论阴虚体质可加速肿瘤的生长,其机理可能是增加肿瘤细胞中的PCNA基因的表达。

Effect of Yin Deficient in Tumor Cell Proliferation of Tumor Bearing Mice

Objective To elucidate the influence of "Yin" and "Yang" imbalance on tumor occurrence.Methods Thyroxine within reserpine,hydrocortisone were used to produce "Yin" and "Yang" deficient model respectively.Then the model mice were transplanted S180 tumor cells.The level of genes expression was analyzed by PT-PCR.Results The "Yin" deficient group's tumor weight was more than the normal group' s and "Yang" deficient group's,but the difference was not significant(P>0.05).Its PCNA gene expression level that affected tumor proliferation was significantly higher,compared with the normal group and "Yang" deficient group(P<0.05).Conclusion "Yin" deficient physique can accelerate tumor growth,the mechanism maybe that it increases the PCNA gene expression in tumor cells.