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| 人黑色素瘤SCID小鼠高转移模型的筛选和建立 | |
| 引用本文： | 姚明,孔韩卫,戚锦芳,周光兴. 人黑色素瘤SCID小鼠高转移模型的筛选和建立[J]. 实验动物与比较医学, 2001, 21(4): 195-197,225 |
| 作者姓名： | 姚明 孔韩卫 戚锦芳 周光兴 |
| 作者单位： | 1. 上海市肿瘤研究所,上海,200032 2. 复旦大学实验动物部,上海,200032 |
| 基金项目： | 上海市卫生局科技发展基金(99430) |
| 摘 要： | 利用人黑色素瘤scid小鼠皮下移植实验中偶尔发现的肺转移瘤组织，接种至scid小鼠皮下，经体内反复筛选，成功建立了一株人黑色素瘤高转移模型SCI-375，至今在体内筛选2年共21代，表现为移植瘤局部侵润、100%自发肺转移合并79%腋下淋巴结转移，组织病理学观察和染色体分析表明，该模型保持了人黑色素瘤的基本特征。同时，利用筛选的肿瘤组织在体外初步建立了一个相应的细胞系，经体内移植实验证实，细胞系保持了原模型的高转移特性。本模型的建立，为研究肿瘤的转移机理和抗转移药物的筛选提供一个理想模型。 |
| 关 键 词： | 人黑色素瘤 SCID小鼠 肿瘤转移 疾病模型 筛选 |
| 文章编号： | 1004-8448(2001)04-0195-03 |
Selection and Establishment Highly Metastatic Model of Human Melanoma in SCID Mice |
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| YAO Ming ,KONG Han-wei ,QI Jin-fang ,ZHOU Guang-xing. Selection and Establishment Highly Metastatic Model of Human Melanoma in SCID Mice[J]. Laboratory Animal and Comparative Medicine, 2001, 21(4): 195-197,225 | |
| Authors: | YAO Ming KONG Han-wei QI Jin-fang ZHOU Guang-xing |
| Affiliation: | YAO Ming 1,KONG Han-wei 1,QI Jin-fang 1,ZHOU Guang-xing 2 |
| Abstract: | We have successfully established a highly metastatic model of human melanoma in scid mice (SCI-375).The selection of metastasis was obtained by using successively subcutaneous implantation of lungs metastatic tissue in scid mice . The metastatic rates were 100% and 80% respectively in lungs and lymph node from 6-21 passages. The lung metastatic tumors were similar to the original human melanoma in histopathological features and chromosomal karyotype. This metastasis model may be used as a tool for the research on the metastatic mechanism and treatment of the human tumor. |
| Keywords: | Human melamoma Tumor metastasis SCID mice Disease model |
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