HPLC-DAD法同时测定抗风湿类中成药及保健食品中非法添加的13种化学成分

目的：同时检测抗风湿类保健食品中非法添加的13种化学成分。方法：采用高效液相色谱串联二极管阵列检测器法，使用XBridge C18（4.6 mm×250 mm，5μm）色谱柱进行分离，流动相为乙腈-0.02 mol·L-1醋酸铵（含0.075%乙酸），梯度洗脱，流速为1.0 mL·min-1，检测波长为230 nm。结果：各化学成分线性回归方程的相关系数均大于0.9999，平均回收率为95.6%～102.5%， RSD均小于1.6%（n=3），并用UPLC-QTOF-MS法进一步确证。结论：该方法操作简便可靠，能同时测定抗风湿类保健食品中非法添加的13种化学成分，分别为对乙酰氨基酚、阿司匹林、甲氧苄啶、氨基比林、氢化可的松、吡罗昔康、地塞米松、萘普生、醋酸泼尼松、双氯芬酸钠、吲哚美辛、保泰松、布洛芬。

Simultaneous Determination of thirteen Chemical Materials Illegally Added in Antirheumatic Traditional Chinese Medicine and Health Products by HPLC-DAD

Objective: To simultaneously detect 13 anti-rheumatic chemical components added illegally in health food. Methods: Using HPLC-DAD, the separation was performed on the XBridge C18 (4.6 mm×250 mm, 5μm) column by gradient elution at a flow rate of 1.0 mL·min-1. The mobile phase consisted of ace-tonitril-0.02 mol·L-1 ammonium acetate (including 0.075% acetic acid) and the detection wavelength was 230 nm. Results: The correlation coefficients of the equation of linear regression were above 0.9999. The average recoveries were 95.6%-102.5% and the relative standard deviations were less than 1.6% (n=3). U-PLC-QTOF-MS was used to further confirm. Conclusion: The method is simple and reliable, and can be used for simultaneously detecting 13 anti-rheumatic chemical components (acetaminophen, aspirin, trimetho-prim, aminopyrine, hydrocortisone, piroxicam, dexamethasone, naproxen, prednisone acetate, diclofenac sodi-um, indometacin, phenylbutazone and ibuprofen) in health food.