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| 桔梗总皂苷含量测定方法研究 | |
| 引用本文： | 李妍,张崇禧,魏建和,许旭东,褚庆龙. 桔梗总皂苷含量测定方法研究[J]. 时珍国医国药, 2009, 20(7) |
| 作者姓名： | 李妍 张崇禧 魏建和 许旭东 褚庆龙 |
| 作者单位： | 1. 吉林农业大学中药材学院,吉林,长春,130118;中国医学科学院中国协和医科大学药用植物研究所,北京,100193 2. 吉林农业大学中药材学院,吉林,长春,130118 3. 中国医学科学院中国协和医科大学药用植物研究所,北京,100193 |
| 基金项目： | 国家自然科学基金面上项目 |
| 摘 要： | 目的 建立一种香草醛-硫酸比色法测定桔梗中总皂苷含量的方法,为桔梗的质量控制提供科学依据.方法 索氏提取器提取,大孔吸附树脂纯化,香草醛-硫酸比色法测定桔梗总皂苷含量.结果 比色法测定的线性方程为A=0.003 4C+0.006 4,相关系数为r=0.999 7,平均加样回收率为96.97%,RSD=1.51%(n=6).对不同来源桔梗中总皂苷含量进行测定,其含量范围为1.07%～3.76%.结论 该法测定准确,重复性好,可用于桔梗药材中桔梗总皂苷的含量测定,便于对桔梗药材进行质量评价. |
| 关 键 词： | 桔梗 总皂苷 桔梗皂苷D 比色法 |
Determination of Total Saponins in Radix Platycodonis |
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| LI Yan,ZHANG Chong-xi,WEI Jian-he,XU Xu-dong,CHU Qing-long. Determination of Total Saponins in Radix Platycodonis[J]. Lishizhen Medicine and Materia Medica Research, 2009, 20(7) | |
| Authors: | LI Yan ZHANG Chong-xi WEI Jian-he XU Xu-dong CHU Qing-long |
| Abstract: | Objective To develop a vanillin -H2SO4 colorimetry method for the determination of total saponins in Radix Platycodonis samples,in order to provide a scientific basis for the quality control of Radix Platycodonis.Methods With Platycodin D as standard,the total saponins in Radix platycodonis was extracted with soxhlet extractor and purified with macroporous resin column chromatography,its content was determined by vanillin -H2SO4 colorimetry method.Results The calibration curve of total saponins in Radix Platycodonis samples for this method was A=0.003 4C+0.006 4,r=0.999 7.The average recovery and RSD were 96.97% and 1.51%,respectively.The content range of total saponins in Radix platycodonis from different origins was from 1.07% to 3.76%.Conclusion This determination method is practical,reproducible and can be used for evaluating the quality of Radix Platycodonis samples. |
| Keywords: | Radix Platycodonis Total saponins Platycodin D Colorimetry |
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