结核感染T细胞斑点试验在脊柱结核诊断中的应用

目的：探讨结核感染T细胞斑点试验在脊柱结核诊断中的临床应用价值。方法选取2012年4月～2013年1月本院骨科病区收治的疑似脊柱结核患者156例，分别采用结核感染T细胞斑点试验和结核菌素试验进行诊断，比较两者对脊柱结核诊断的临床意义（包括灵敏性、特异性、假阳性率和假阴性率指标的差别）。结果结核感染T细胞斑点试验的灵敏性和特异性明显高于结核菌素试验（P＜0．05）；同时，结核感染T细胞斑点试验的假阳性率和假阴性率明显低于结核菌素试验试验（P＜0．05）。结论结核感染T细胞斑点试验在诊断脊柱结核中具有良好的敏感性和特异性，具有重要临床应用价值。

Primary study of T-cell spot of tuberculosis test in diagnosis of spinal tuberculosis

Objective To investigate the diagnostic value of T-cell spot of tuberculosis(T-SPOT.TB) test in patients with spinal tuberculosis.Methods The recorded clinical data of 156 suspected patients with spinal tuberculosis during April 2012 to January 2013,were collected retrospectively and reviewed systematically by the T-SPOT.TB test and tuberculin skin test(TST). The sensitivity, specificity, false positive rate and false negative rate of 2 tests were compared.Results The sensitivity and specificity of T-SPOT.TB test was significantly higher than those of TST test(P<0.05);the false positive rate and false negative rate of T-SPOT.TB test were significantly lower than those of TST test(P<0.05).Conclusion T-SPOT.TB assay possesses satisfactory sensitivity and specificity in diagnosis of spinal tuberculosis, and it is an effective way for the diagnosis of spinal tuberculosis.