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H3N2 Influenza A Virus Replicates in Immortalized Human First Trimester Trophoblast Cell Lines and Induces Their Rapid Apoptosis |
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| Authors: | Quang Duy Trinh Yasuyuki Izumi Shihoko Komine-Aizawa Toshikatsu Shibata Yoshitaka Shimotai Kazumichi Kuroda Masashi Mizuguchi Hiroshi Ushijima Gil Mor Satoshi Hayakawa |
| Affiliation: | Division of Microbiology, Department of Pathology and Microbiology, Nihon University School of Medicine, Tokyo, Japan;; Department of Developmental Medical Sciences, Institute of International Health, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan;; Aino Health Science Center, Aino University, Tokyo, Japan;; Department of Obstetrics, Gynecology and Reproductive Sciences, Reproductive Immunology Unit, Yale University School of Medicine, New Haven, CT, USA |
| Abstract: | Problem Epidemiological data suggested that pandemic influenza increased the risks of spontaneous abortion and premature labor, while seasonal influenza also increased the risk of schizophrenia in adolescence. However, their pathogenesis is so far unknown. Method of study The first trimester trophoblast cell lines, namely, Swan71 and HTR8 cells were challenged with A/Udorn/72 influenza virus (H3N2). At indicated time points, cells were examined for expression of influenza proteins. Viral replication in culture media, apoptosis and the expression of human leukocyte antigen (HLA)-G were also examined. Results Intracellular localization of viral proteins was observed. Twenty-four hours after inoculation, virus was detected in culture media while most cells fell into apoptosis. During apoptosis, expression of HLA-G was unchanged. Conclusion We revealed replication of low pathogenic influenza virus in the first trimester trophoblast cell lines. Placental damages are likely to be induced by direct cytopathic effects of influenza virus and subsequent apoptosis rather than down regulation of HLA-G expression and subsequent rejection by maternal immune system. |
| Keywords: | Apoptosis first trimester trophoblast influenza A virus |