Human papillomavirus was not detected by PCR using multiple consensus primer sets in esophageal adenocarcinomas in Chinese patients |
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| Authors: | Songtao Feng Jianming Zheng Xiang Du Yunshan Tan Haijun Yang Hongxia Zhang Zhigang Zhang |
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| Affiliation: | 1. Department of Nephrology, The Affiliated People's Hospital of Jiangsu University, Zhenjiang, Jiangsu Province, China;2. Department of Pathology, Shanghai Medical College, Fudan University, Shanghai, China;3. Department of Pathology, Changhai Hospital, The Second Military Medical University, Shanghai, China;4. Department of Pathology, Shanghai Cancer Hospital, Fudan University, Shanghai, China;5. Department of Pathology, Zhongshan Hospital, Fudan University, Shanghai, China;6. Department of Pathology, Anyang Tumor Hospital, Anyang, Henan Province, China |
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| Abstract: | ![]()
The role of human papillomavirus (HPV) infection in the development of esophageal squamous cell carcinoma is well established; however, there are few reports on the role of HPV in esophageal adenocarcinoma. To evaluate the putative role of HPV infection in esophageal adenocarcinoma, 57 formalin‐fixed, paraffin‐embedded esophageal adenocarcinoma specimens were collected from four hospitals in Shanghai and Anyang, China, between 1999 and 2008. HPV DNA was analyzed using PCR with multiple sets of consensus primers for HPV, GP5+/6+, CPI/CPIIG, SPF10, pU‐1M/pU2R, and pU31B/pU2R. Glyceraldehyde‐3‐phosphate dehydrogenase (GAPDH), the internal control, was amplified successfully in all 57 specimens. However, HPV amplification was not detected in any specimens with any of the consensus primer sets used. The present study indicates that HPV infection is not likely to be a major factor in the etiology of esophageal adenocarcinoma in the Chinese population. J. Med. Virol. 85: 1053–1057, 2013. © 2013 Wiley Periodicals, Inc. |
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| Keywords: | human papillomavirus multiple consensus primer sets esophageal adenocarcinoma |
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