microRNA-25高表达与直肠癌细胞的侵袭和迁移的关系

目的:探讨micro RNA-25(mi R-25)在直肠癌细胞中的表达及作用。方法:检测多种不同直肠癌细胞中mi R-25的表达,并检测直肠癌细胞转染mi R-25前体(pre-mi R-25)上调mi R-25的表达或转染mi R-25抑制剂(anti-mi R-25)下调mi R-25的表达后生物学行为的变化。结果:与正常直肠黏膜组织比较,不同的直肠癌细胞中mi R-25的表达均不同程度的明显升高(均P0.05)。在mi R-25表达水平相对较低的直肠癌HR-834细胞中转染pre-mi R-25,在mi R-25高表达的直肠癌SW-837细胞中转染anti-mi R-25后,两种细胞的增殖、细胞周期、凋亡无明改变(均P0.05),但侵袭及迁移能力在HR-834细胞的明显增强,SW-837细胞减弱(均P0.05)。结论:mi R-25在直肠癌细胞中的表达升高,且其升高程度与细胞的侵袭和迁移能力密切相关。

Relations of high microRNA-25 expression with migration and invasion of rectal carcinoma cells

Objective: To investigate microRNA-25 (miR-25) expression in rectal carcinoma cells and its action. Methods: The miR-25 expressions in several different rectal cell lines were determined, and then the changes in biological behaviors in rectal cancer cells after up- or down-regulation of miR-25 expression with miR-25 precursor (pre-miR-25) or miR-25 inhibitor (anti-miR-25) transfection were examined. Results: Compared with normal rectal mucosal tissue, the miR-25 expressions in all tested rectal cancer cells were significantly increased with varying degrees (all P<0.05). In rectal cancer HR-834 cells with relatively low miR-25 expression after pre-miR-25 transfection and in rectal cancer SW-837 cells with high miR-25 expression after anti-miR-25 transfection, the proliferation, cell cycle and apoptosis showed no significant change (all P<0.05), but the invasion and migration abilities in HR-834 cells were increased, and SW-837 cells were decreased significantly (all P<0.05). Conclusion: The miR-25 expression is elevated in rectal cancer cells, and its degree of increase is closely related to the invasion and migration abilities of rectal cancer cells.