| 深低温长期保存兔角膜内皮细胞体外培养的实验研究 |
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| 引用本文： | 崔向东,王传富,于华英.深低温长期保存兔角膜内皮细胞体外培养的实验研究[J].齐鲁医学杂志,1999,14(1):6. |
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| 作者姓名： | 崔向东 王传富 于华英 |
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| 作者单位： | 青岛医学院附属医院 |
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| 摘 要： | ①目的观察深低温保存后兔角膜内皮细胞的活性。②方法将深低温保存兔角膜与新鲜兔角膜进行内皮细胞体外培养对比观察。③结果在培养７ｄ内，深低温保存的角膜内皮细胞较新鲜角膜内皮细胞滞后１～２ｄ生长，１４ｄ以后二者逐渐趋于一致。④结论经深低温长期保存的兔角膜内皮细胞仍保持良好的生长活性
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| 关 键 词： | 角膜 低温保存 细胞，培养的 兔 |
CELL CULTURE OF ENDOTHELIAL CELLS OF CRYOPRESERVED RABBIT CORNEA |
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| Cui Xiangdong,Wang Chuanfu,Yu Huaying,et al.CELL CULTURE OF ENDOTHELIAL CELLS OF CRYOPRESERVED RABBIT CORNEA[J].Medical Journal of Qilu,1999,14(1):6. |
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| Authors: | Cui Xiangdong Wang Chuanfu Yu Huaying |
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| Institution: | Cui Xiangdong,Wang Chuanfu,Yu Huaying,et al Department of Cryomedicine,The Affiliated Hospital of Qingdao Medical College,Qingdao 266003 |
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| Abstract: | Objective To determine the activities of endotheliocytes of cryopreserved rabbit cornea.\ Methods\ The endotheliocytes of both cryopreserved and fresh rabbit cornea were cultured.\ Results\ During first 7 days of cell culture, the growth of endotheliocytes of the cryopreserved cornea delayed 1-2 days compared with that of the fresh cornea. After 14 days of in vitro culture, both cryopreserved and fresh rabbit cornea growed similarly.\ Conclusion\ Endothelial cells of the long term cryopreserved rabbit corneas maintained good activities. |
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| Keywords: | cornea cryopreservation culture rabbit |
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