石杉碱甲对 D-半乳糖致衰老小鼠学习记忆行为的影响

背景石杉碱甲是中国首创的一种可逆的胆碱酯酶抑制剂,其对良性记忆障碍有较好的疗效,能明显促进啮齿类动物的学习过程和记忆再现. 目的了解石杉碱甲对D-半乳糖拟衰老模型小鼠在跳台及迷宫实验中增强学习记忆行为的作用.设计随机对照实验研究. 单位新乡医学院药物研究室. 材料实验在新乡医学院生理实验室及药物研究室完成.选择健康昆明种小鼠100只用跳台法实验和电迷宫法实验,每个实验组各 50只.石杉碱甲片 ,由河南竹林众生制药股份有限公司提供,批号 031001;D 半乳糖 ,上海试剂二厂出品. 干预 D半乳糖对照组和石杉碱甲治疗组建立亚急性衰老模型 每日颈背部皮下注射120 mg/(kg· d)D-半乳糖 ,连续 6周 ,空白对照注射等量生理盐水.同时在以上处理的第37天开始 ,3个石杉碱甲治疗组分别用 0.2 mg/kg, 0.4 mg/kg, 0.8 mg/kg的 3种不同剂量灌胃,空白对照组和 D-半乳糖对照组以等量生理盐水灌胃 ,连续 7 d.主要观察指标跳台法主要观察小鼠在跳台上安全区的潜伏时间和 5min内小鼠触电次数;电迷宫实验主要观察小鼠逃至右侧电击区的错误次数.结果跳台法检测结果显示, D半乳糖致衰老模型小鼠的潜伏期时间较短,错误次数较多.空白对照组[(9.2± 38.7) s,(4.12± 1.02)次 ]与 D-半乳糖对照组 [(49.7± 27.1) s,(5.17± 0.64)次]比较,差异有显著性( P< 0.05).石杉碱甲治疗组小鼠的潜伏期时间明显延长、错误次数明显减少,差异更明显(P< 0.01).②电迷宫实验结果显示, D-半乳糖致衰老小鼠逃至右侧电击区的次数明显增加.与D 半乳糖对照组 [(7.02± 1.06)次 ]比较,空白对照组错误次数 [(6.05± 0.70)次]减少( P< 0.05),而石杉碱甲治疗组更明显( P< 0.01),且中等剂量( 0.4mg/kg)治疗组错误次数更少 [(3.86± 1.80)次 ]. 结论石杉碱甲能有效提高D-半乳糖致衰老小鼠的学习记忆能力;在一定范围内显示出量效关系.

Effects of Huperzine A on the memory and learning behavior in D galactose induced senile mice

BACKGROUND:Huperzine A is a reversible choline enzyme(CHE) inhibitor initially developed in China,which has favorable therapeutic effectiveness in benign memory dysfunction,and could significantly promote the learning process and memory reappearance in rodent animals. OBJECTIVE:To comprehend the impacts of Huperzine A on the enhancement of learning, memory and behavior in platform and maze experiments in D Galoctose(Gal) induced senile mouse model. DESIGN:A randomized and controlled trial. SETTING:Institute of Pharmaceuticals of Xinxiang Medical College. MATERIALS:Study was performed in the Laboratory of Physiology and the Institute of Pharmaceuticals of Xinxiang Medical College from January to December in 2002.A total of 100 healthy Kunming mice were selected for platform and electric maze experiments with 50 mice in each experiment.Huperzine A tablet was obtained from Henan Zhulin Zhongsheng Pharmaceutical Company Limited(batch number:031001).D Gal is the product of Shanghai No.2 Reagent Factory. MAIN OUTCOME MEASURES:The latency in the safe area on the platform and the times of electric shock occurred within 5 minutes were observed in mice in platform experiment;the times of the mice mistakenly escaping to the right electric attacking area were observed in mice in electric maze experiment. CONCLUSION:Huperzine A can effectively improve the learning and memory ability of D Gal induced senile mice, and there is dose effect relationship within certain range. Qiu PY,Chen ZY,Wang YX,Zhang JX,Wu ZZ.Effects of Huperzine A on the memory and behavior in D galactose induced senile mice.Zhongguo Linchuang Kangfu 2005;9(4):247- 9(China) [www.zglckf.com]