| Chemical and enzymatic synthesis and doning of human epidermal growth factor gene |
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| 引用本文： | 赵忠良,陈南春,李飚,陈苏民. Chemical and enzymatic synthesis and doning of human epidermal growth factor gene[J]. 中国人民解放军军医大学学报, 1992, 0(2) |
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| 作者姓名： | 赵忠良 陈南春 李飚 陈苏民 |
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| 作者单位： | Department of Biochemistry Fourth Military Medical University，Xi'an 710032，Department of Biochemistry，Fourth Military Medical University，Xi'an 710032，Department of Biochemistry，Fourth Military Medical University，Xi'an 710032，Department of Biochemistry，Fourth Military Medical University，Xi'an 710032 |
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| 摘 要： | ![]()
The human epidermal growth factor gene was synthesized with DNA synthesizer andby enzymes.Computer assisted to design the gene sequence and the restriction enzyme sites,andto elhninate the repeated and self-complementary sequences.So that multiple doning and gene ex-pression were available for the designed gene sequence.The whole gene was formed by annealing8 chemically synthesized oligodeoxynucleotide fragments,enzymatically filling up the 4 twenty-basespaces and ligating the nicks,and was inserted into EcoR I and HInc Ⅱ sites ofplasmid pUC12.The recombinant plasmid was transformed into E.coli JM103 and 200 whitedones were obtained by X-gal and ampicilin screening.The positive clones were isolated andcharacterized by restriction enzyme analysis and DNA sequencing.
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Chemical and enzymatic synthesis and doning of human epidermal growth factor gene |
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| Zhao Zhongliang,Chen Nanchun,Li Biao,Chen Sumin. Chemical and enzymatic synthesis and doning of human epidermal growth factor gene[J]. Journal of Medical Colleges of PLA(China), 1992, 0(2) |
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| Authors: | Zhao Zhongliang Chen Nanchun Li Biao Chen Sumin |
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| Affiliation: | Zhao Zhongliang,Chen Nanchun,Li Biao,Chen Sumin Department of Biochemistry,Fourth Military Medical University,Xi'an 710032 |
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| Abstract: | ![]()
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| Keywords: | epidermal growth factor DNA recombinant gene synthesis human |
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