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| 华蟾素诱导HL-60细胞凋亡及机制研究 | |
| 引用本文： | 霍英,徐华丽,孙德军. 华蟾素诱导HL-60细胞凋亡及机制研究[J]. 中国药师, 2008, 11(10) |
| 作者姓名： | 霍英 徐华丽 孙德军 |
| 作者单位： | 1. 吉林大学再生医学科学研究所生物技术药物教研室,长春,130021 2. 吉林大学药学院药理教研室 |
| 摘 要： | 目的：观察华蟾素（cinobufacini）对白血病HL-60细胞的增殖抑制作用和诱导凋亡作用的机制。方法：以HL-60细胞为研究对象，采用MTT法观察细胞增殖作用；Annexin V-FITC／PI双染和吖啶橙／溴乙锭（AO／EB）荧光染色法检测细胞凋亡；罗丹明染色法检测线粒体膜电位；分光光度法检测Caspase-3活性。结果：在0．78～6．25μg·ml^-1，华蟾素能明显的抑制HL-60细胞的增殖；华蟾素作用24h后，AnnexinV阳性的细胞从7．81％增加至66．02％，而且在荧光显微镜下可以明显观察到凋亡细胞；线粒体膜电位下降和Caspase-3活性升高。结论：华蟾素能够抑制HL-60细胞增殖，这种作用与其诱导细胞凋亡破坏线粒体功能和激活Caspase-3有关。 |
| 关 键 词： | 华蟾素 HL-60 细胞凋亡 Caspase-3 |
Effects of Cinobufacini Oil Apoptosis and Mechanism in HL-60 cells |
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| Huo Ying,Xu Huali,Sun Dejun. Effects of Cinobufacini Oil Apoptosis and Mechanism in HL-60 cells[J]. China Pharmacist, 2008, 11(10) | |
| Authors: | Huo Ying Xu Huali Sun Dejun |
| Affiliation: | Huo Ying~1 Xu Huali~2 Sun Dejun~1(1.Institute of Frontier Medical Science,Jilin University,Changchun 130021,China,2.Department of Pharmacology,School of Pharmacy,Jilin University) |
| Abstract: | Objective:To investigate the effects of proliferation inhibitory and the molecule mechanism of apoptosis induced by einobufacini on HL-60 cells.Method:Human leukemia HL-60 cells were used.The effects of einobufacini on cell proliferation were observed by MTF method in vitro.The apoptosis was evaluated by Annexin V-FITC/PI and AO/EB fluorescent staining.Mitochondrial membrane potential and Caspase-3 were measured.Result:Cinobufacini exhibited a marked antiproliferativc effect on HL-60 cells within the concen... |
| Keywords: | HL-60 Caspase-3 |
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