眼镜蛇毒高活性抗补体因子的体外溶血活性研究

目的　了解云南孟加拉眼镜蛇毒高活性抗补体因子的体外溶血活性。方法　采用由豚鼠血清、豚鼠红细胞和该抗补体因子组成的体外溶血体系 ,对其溶血活性、溶血活性稳定性以及多种二价金属离子、EDTA对其溶血活性的影响进行研究。结果　该抗补体因子溶血活性的比活力为 1 391KU·g- 1 ,其溶血活性对温度、酸碱表现出较好的稳定性 ,1、2、5mmol·L- 1 的Ca2 + 、Mg2 + 、Mn2 + 能促进抗补体因子的溶血活性 ,1、2mmol·L- 1 的Zn2 + 、Co2 + 能促进抗补体因子的溶血活性 ,而 5mmol·L- 1 的Zn2 + 、Co2 + 则抑制抗补体因子的溶血活性 ,Cu2 + 在 1、2、5mmol·L- 1 时则强烈抑制抗补体因子的溶血活性 ,EDTA能强烈抑制抗补体因子的溶血活性。结论　云南孟加拉眼镜蛇毒高活性抗补体因子具有一定的溶血活性 ,并且具有较好的温度耐受性及酸碱稳定性。多种二价金属离子及EDTA能促进或抑制这种溶血活性

In vitro hemolyzation of a highly active anticomplement factor from the venom of Naja kaouthia

AIM To study the hemolyzation of a highly active anti complementary protein (cobra venom factor, CVF) isolated from the venom of Naja kaouthia distributed in the south of Yunnan Province, China. METHODS Guinea pig red blood cell and serum were employed to evaluate the hemolyzation of this anticomplement factor, and the effects of temperature, pH, various bivalent mental ions and EDTA on its hemolyzation were investigated. RESULTS This anticomplement factor possessed hemolytic activity of 1 391 kU·g -1 protein. It showed high stability to heat, alkalinity and acidity. Ca 2+ , Mg 2+ , Mn 2+ promoted the hemolyzation of this anticomplement factor at 1, 2 and 5 mmol·L -1 . Zn 2+ , Co 2+ also promoted the hemolyzation of this anticomplement factor at 1 and 2 mmol·L -1 , but inhibited the hemolyzation at 5 mmol·L -1 . Cu 2+ strongly inhibited the hemolyzation at 1, 2 and 5 mmol·L -1 . EDTA also strongly inhibited the hemolyzation of this anticomplement factor. CONCL- USION The anticomplement factor showed hemolytic activity. This ability could be influenced by bivalent metal ions and EDTA, but rather stable when temperature or pH value changed.